{"id":1794,"date":"2017-04-04T13:42:00","date_gmt":"2017-04-04T11:42:00","guid":{"rendered":"https:\/\/www.fractioncollector.info\/?p=1794"},"modified":"2024-06-27T17:55:49","modified_gmt":"2024-06-27T15:55:49","slug":"perfuze-ostruvku-s-automatizovanym-sberacem-frakci-omnicoll","status":"publish","type":"post","link":"https:\/\/www.fractioncollector.info\/cs\/islets-perifusion-with-automated-fraction-collector-omnicoll\/","title":{"rendered":"Perif\u00faze ostr\u016fvk\u016f s automatizovan\u00fdm sb\u011bra\u010dem frakc\u00ed"},"content":{"rendered":"

PERIFUZE OSTR\u016eVK\u016e: Automatizovan\u00fd v\u00edcekan\u00e1lov\u00fd sb\u011br frakc\u00ed pomoc\u00ed LAMBDA OMNICOLL<\/span><\/strong><\/h1>\n<\/div><\/div><\/div><\/div><\/div>
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\n\t\t\t\t\t\tOSTR\u016eVKY SLINKOVICE\t\t\t\t\t\t<\/h2>\n\t\t\t\t\t\t<\/div>\n<\/div><\/div><\/div>
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Pankreatick\u00e9 ostr\u016fvky<\/strong> se p\u0159edpokl\u00e1d\u00e1, \u017ee hraj\u00ed kl\u00ed\u010dovou roli v patofyziologii Diabetes 1. a 2. typu<\/strong> kv\u016fli neschopnosti \u03b2-bun\u011bk ostr\u016fvk\u016f vylu\u010dovat dostate\u010dn\u00e9 mno\u017estv\u00ed inzul\u00ednu k regulaci hladiny gluk\u00f3zy v krvi.<\/p>\n

Definitivn\u00ed m\u00edrou kvality \u03b2-bun\u011bk v ostr\u016fvc\u00edch je m\u011b\u0159en\u00ed funkce \u03b2-bun\u011bk, tj. schopnosti ostr\u016fvk\u016f kontrolovan\u011b uvol\u0148ovat inzul\u00edn v reakci na nepatrn\u00e9 zm\u011bny hladiny gluk\u00f3zy v okol\u00ed.<\/p>\n

Vyu\u017eit\u00ed perfuzn\u00edch syst\u00e9m\u016f k pochopen\u00ed kinetiky \u03b2-bun\u011bk in vitro bylo kl\u00ed\u010dov\u00fdm faktorem pro objasn\u011bn\u00ed fyziologie a patofyziologie ostr\u016fvk\u016f. Perif\u00faze ostr\u016fvk\u016f zahrnuje vystaven\u00ed ostr\u016fvk\u016f nep\u0159etr\u017eit\u00e9mu toku \u017eivin, plynu nebo \u010dinidel<\/strong>. N\u011bkolik v\u00fdzkumn\u00edk\u016f publikovalo perfuzn\u00ed syst\u00e9my s regulac\u00ed teploty, pH a pr\u016ftoku perifuz\u00e1tu.<\/p>\n

V sou\u010dasn\u00e9 dob\u011b neexistuje standardizovan\u00fd, levn\u00fd p\u0159\u00edstroj a protokol, kter\u00fd by bylo mo\u017en\u00e9 snadno replikovat v jak\u00e9koli laborato\u0159i.<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div>

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\n\t\t\t\t\t\tPERIFUZE OSTR\u016eVK\u016e\t\t\t\t\t\t<\/h2>\n\t\t\t\t\t\t<\/div>\n<\/div><\/div><\/div>
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Ten\/Ta\/To nastaven\u00ed perfuze ostr\u016fvk\u016f<\/strong> se skl\u00e1d\u00e1 z \u010dty\u0159i perfuzn\u00ed kolony<\/strong> p\u0159ipojeno ke \u010dty\u0159kan\u00e1lov\u00fdm pump\u00e1m Isamtec, pro sou\u010dasn\u00e9 \u010derp\u00e1n\u00ed 4 r\u016fzn\u00fdch perfuzn\u00edch roztok\u016f do 4 paraleln\u00edch kolon obsahuj\u00edc\u00edch ostr\u016fvky s pr\u016ftok 100 \u00b5l\/min<\/strong>. Kontinu\u00e1ln\u00ed tok nebo dynamick\u00e1 perfuze roztoku obsahuj\u00edc\u00edho gluk\u00f3zu a sekretagogy skrz ostr\u016fvky je nejp\u0159esn\u011bj\u0161\u00edm zp\u016fsobem hodnocen\u00ed regulovan\u00e9ho uvol\u0148ov\u00e1n\u00ed inzul\u00ednu in vitro.<\/p>\n

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Automatizovan\u00fd v\u00edcekan\u00e1lov\u00fd sb\u011bra\u010d frakc\u00ed LAMBDA OMNICOLL umo\u017e\u0148uje pou\u017eit\u00ed peristaltick\u00fdch \u010derpadel t\u0159et\u00edch stran s analogov\u00fdmi sign\u00e1ly.\u00a0<\/em><\/p>\n<\/blockquote>\n

\"Perif\u00faze<\/p>\n

Kolony s ostr\u016fvky a perfuzn\u00edm m\u00e9diem byly udr\u017eov\u00e1ny p\u0159i teplot\u011b 37 \u00b0C. v komo\u0159e s \u0159\u00edzenou teplotou.<\/strong> Ostr\u016fvky byly perfuzov\u00e1ny po dobu 10\u201315 minut na stimul, aby se umo\u017enila jejich spr\u00e1vn\u00e1 expozice a sb\u011br jejich sekre\u010dn\u00edch produkt\u016f.<\/p>\n

Perifuz\u00e1ty (100 \u03bcL\/min) ze 4 kolon byly odeb\u00edr\u00e1ny sou\u010dasn\u011b ka\u017edou minutu automatizovan\u00fdm 4kan\u00e1lov\u00fd sb\u011bra\u010d frakc\u00ed LAMBDA OMNICOLL do 96jamkov\u00fdch desti\u010dek<\/strong>. \u010cty\u0159kan\u00e1lov\u00fd sb\u011bra\u010d vzork\u016f LAMBDA OMNICOLL ka\u017edou minutu vyb\u00edr\u00e1 4 horn\u00ed jamky 96jamkov\u00e9 desti\u010dky. (A, B, C a D \u2013 ka\u017ed\u00fd p\u0159edstavuje jin\u00fd l\u00e9\u010debn\u00fd stav)<\/em> shrom\u00e1\u017edit 12 minut zlomk\u016f a pot\u00e9 se p\u0159esunout do spodn\u00edch \u010dty\u0159 \u0159\u00e1dk\u016f (\u0158ady E, F, G a H)<\/em> 96jamkov\u00e9 desti\u010dky.<\/p>\n

Perifuz\u00e1t ve sb\u011brn\u00fdch 96jamkov\u00fdch desti\u010dk\u00e1ch byl uchov\u00e1v\u00e1n p\u0159i teplot\u011b <4 \u00b0C, aby se zachovala integrita analyt\u016f.<\/p>\n

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V\u00edcekan\u00e1lov\u00e9 z\u00e1kladny sb\u011bra\u010d\u016f frakc\u00ed LAMBDA OMNICOLL (obsahuj\u00edc\u00ed vzorky perifuz\u00e1tu) lze um\u00edstit do studen\u00e9 l\u00e1zn\u011b nebo jak\u00e9koli jin\u00e9 termostabilizovan\u00e9 n\u00e1doby.<\/em><\/p>\n<\/blockquote>\n

Odebran\u00e9 vzorky perifuz\u00e1tu byly testov\u00e1ny na koncentrace inzulinu, glukagonu nebo somatostatinu<\/strong>. M\u011b\u0159en\u00ed r\u016fzn\u00fdch hormon\u016f lze prov\u00e1d\u011bt v r\u016fzn\u00fdch jamk\u00e1ch stejn\u00e9 96jamkov\u00e9 desti\u010dky.
\nV principu 96 vzork\u016f z p\u0159\u00edpravy v\u00edce ostr\u016fvk\u016f<\/strong> s r\u016fzn\u00fdmi experiment\u00e1ln\u00edmi podm\u00ednkami lze m\u011b\u0159it sou\u010dasn\u011b, co\u017e umo\u017e\u0148uje vysoce v\u00fdkonn\u00fd screening.<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div>

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Automatizovan\u00fd v\u00edcekan\u00e1lov\u00fd sb\u011br frakc\u00ed s LAMBDA OMNICOLL konfigurovan\u00fdm pro 96jamkov\u00e9 desti\u010dky<\/h2>\n

Pro 96jamkov\u00e9 desti\u010dky d\u00e1le automatizovan\u00e9 v\u00edcekan\u00e1lov\u00e9 sb\u011bra\u010de frakc\u00ed LAMBDA OMNICOLL<\/a> p\u0159izp\u016fsobil\/a:<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div>

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\"Zak\u00e1zkov\u00fd<\/p>\n

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NOVINKA! Konfigura\u010dn\u00ed verze 2022<\/strong><\/a>
\nP\u0159\u00edklad:<\/em> Zak\u00e1zkov\u00fd 6kan\u00e1lov\u00fd sb\u011bra\u010d frakc\u00ed LAMBDA OMNICOLL pro 96jamkov\u00e9 desti\u010dky.<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div>

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Dal\u0161\u00ed p\u0159\u00edklady: \"Sb\u011br<\/p>\n

Sb\u011br zlomk\u016f s 4 kan\u00e1ly<\/strong> do 96jamkov\u00fdch desti\u010dek pro perfuzi ostr\u016fvk\u016f<\/p>\n<\/div>\n<\/div><\/div><\/div>

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\"Automatizovan\u00fd<\/p>\n

Automatizovan\u00fd v\u00edcekan\u00e1lov\u00fd sb\u011bra\u010d frakc\u00ed pro 6 simult\u00e1nn\u00edch stream\u016f<\/strong> (1x6) do 96jamkov\u00fdch desti\u010dek<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div>

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\"V\u00edcekan\u00e1lov\u00fd<\/p>\n

P\u0159izp\u016fsoben\u00fd v\u00edcekan\u00e1lov\u00fd automatick\u00fd vzorkova\u010d LAMBDA OMNICOLL s 2 simult\u00e1nn\u00ed streamy<\/strong> a dv\u011b LAMBDA \u010derpadla<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div><\/div>","protected":false},"excerpt":{"rendered":"

PERIFUZE OSTR\u016eVK\u016e: Automatizovan\u00fd v\u00edcekan\u00e1lov\u00fd sb\u011br frakc\u00ed pomoc\u00ed LAMBDA OMNICOLL<\/p>","protected":false},"author":2,"featured_media":1798,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"wpai_generated_summary":"","footnotes":""},"categories":[132],"tags":[95,138,139,137,119,140],"class_list":["post-1794","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-post-name","tag-4-channel","tag-96-well-plates","tag-automated-fraction-collector","tag-islets-perifusion","tag-ismatec-multichannel-pump","tag-pancreatic-islets","post-with-thumbnail","post-with-thumbnail-icon"],"yoast_head":"\nIslets perifusion with automated fraction collector – LAMBDA OMNICOLL | LAMBDA Laboratory Instruments<\/title>\n<meta name=\"description\" content=\"Islets perifusion set-up with an automated 4-channel fraction collector OMNICOLL, to collect perifusate into 96-well 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