{"id":1707,"date":"2017-01-10T14:44:45","date_gmt":"2017-01-10T12:44:45","guid":{"rendered":"https:\/\/www.fractioncollector.info\/?p=1707"},"modified":"2024-08-05T16:03:04","modified_gmt":"2024-08-05T14:03:04","slug":"fraction-collector-protein-purification-chromatography","status":"publish","type":"post","link":"https:\/\/www.fractioncollector.info\/de\/fraction-collector-protein-purification-chromatography\/","title":{"rendered":"Successful Protein Purification: Gradient elution"},"content":{"rendered":"<div id=\"pl-1707\"  class=\"panel-layout\" ><div id=\"pg-1707-0\"  class=\"panel-grid panel-has-style\"  style=\"margin-bottom: 50px\" ><div class=\"siteorigin-panels-stretch panel-row-style-full-width panel-row-style panel-row-style-for-1707-0\" style=\"padding: 0px 0px 0px 0px;\" data-stretch-type=\"full-width-stretch\" ><div id=\"pgc-1707-0-0\"  class=\"panel-grid-cell\" ><div id=\"panel-1707-0-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"0\" ><div class=\"textwidget\"><h1 style=\"text-align: center;\"><span style=\"color: #ffffff; font-size: 35px; text-shadow: 1px 1px #000000;\">Protein purification: Liquid chromatography &amp; gradient elution<\/span><\/h1>\n<h2 style=\"text-align: center;\"><span style=\"color: #333333; font-size: 20px; text-shadow: 1px 1px #000000;\">with OMNICOLL fraction collector and LAMBDA peristaltic pumps<\/span><\/h2>\n<\/div><\/div><\/div><\/div><\/div><div id=\"pg-1707-1\"  class=\"panel-grid panel-no-style\"  style=\"margin-bottom: 50px\" ><div id=\"pgc-1707-1-0\"  class=\"panel-grid-cell\" ><div id=\"panel-1707-1-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"1\" ><div class=\"textwidget\"><p>For <strong>protein purification<\/strong> and <strong>isolation of peptides, nucleic acids or small molecules<\/strong> from the crude mixture of fermentation broth, <strong>liquid<\/strong>\u00a0<strong>column chromatography (LC) is widely used<\/strong>\u00a0with ion exchange, hydrophobic interaction, affinity or size exclusion in downstream processing (DSP).<\/p>\n<p>In order to <strong>optimize the liquid chromatography processes<\/strong>, the OMNICOLL fraction collector and reliable LAMBDA peristaltic liquid pumps are successfully used in laboratory practice:<\/p>\n<\/div><\/div><\/div><\/div><div id=\"pg-1707-2\"  class=\"panel-grid panel-no-style\"  style=\"margin-bottom: 50px\" ><div id=\"pgc-1707-2-0\"  class=\"panel-grid-cell\" ><div id=\"panel-1707-2-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"2\" ><div class=\"textwidget\"><h3><span style=\"color: #60351d;\">Example setup of automatic fraction collection<\/span><\/h3>\n<p>For example, a complete <b>arrangement of column chromatography with automatic collection<\/b> of eluate using OMNICOLL fraction collector includes:<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-2635 size-full\" title=\"LAMBDA OMNICOLL fraction collector and LAMBDA peristaltic pumps for protein purification by concentration gradient elution\" src=\"https:\/\/www.fractioncollector.info\/wp-content\/uploads\/OMNICOLL-fraction-collector-LAMBDA-pumps-protein-purification-gradient-elution.jpg\" alt=\"LAMBDA OMNICOLL fraction collector and LAMBDA peristaltic pumps for protein purification by concentration gradient elution\" width=\"700\" height=\"400\" srcset=\"https:\/\/www.fractioncollector.info\/wp-content\/uploads\/OMNICOLL-fraction-collector-LAMBDA-pumps-protein-purification-gradient-elution.jpg 700w, https:\/\/www.fractioncollector.info\/wp-content\/uploads\/OMNICOLL-fraction-collector-LAMBDA-pumps-protein-purification-gradient-elution-418x239.jpg 418w, https:\/\/www.fractioncollector.info\/wp-content\/uploads\/OMNICOLL-fraction-collector-LAMBDA-pumps-protein-purification-gradient-elution-150x86.jpg 150w\" sizes=\"auto, (max-width: 700px) 100vw, 700px\" \/><\/p>\n<ul>\n<li>Two similar containers with <b>elution buffer A &amp; B connected by a siphon<\/b> (or tube at the bottom with a valve to adjust the flow)<\/li>\n<li><b>Elution buffer A<\/b> is consistently drawn into the beaker with <b>elution buffer B<\/b> which is constantly <b>mixed by magnetic stirrer<\/b>, thereby <b>altering its buffer concentration<\/b><\/li>\n<li>Programmable LAMBDA peristaltic pump to <b>pump the gradient mixture<\/b> into the column<\/li>\n<li><b>Eluate from the column<\/b> is being passed through the <b>detector <\/b>(to measure pH, Absorbance 280 nm, conductance)<\/li>\n<li>From the detector, the <b>protein fractionation<\/b> is collected by the <b>OMNICOLL fraction collector<\/b> into the desired containers (e.g.: tubes, vials, etc.)<\/li>\n<\/ul>\n<p>The proteins are eluted by changing the composition of the buffer <i>(ionic strength, pH, conductivity, concentration of salts, etc.)<\/i>. The elution conditions can be changed from linear, stepwise or segmented stepwise by <b>changing the flow of buffer A to B<\/b> in which the mobile phase composition changes over the time.<\/p>\n<\/div><\/div><\/div><\/div><div id=\"pg-1707-3\"  class=\"panel-grid panel-no-style\"  style=\"margin-bottom: 50px\" ><div id=\"pgc-1707-3-0\"  class=\"panel-grid-cell\" ><div id=\"panel-1707-3-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"3\" ><div class=\"textwidget\"><h2 style=\"text-align: center;\">Procedure<\/h2>\n<p>In the liquid column chromatography, it is important to\u00a0pack the column\u00a0evenly<strong> without\u00a0air bubbles, channels, and\u00a0cracks on the stationary phase<\/strong>.<\/p>\n<p>The LAMBDA peristaltic pump supplies the <strong>liquid mobile phase continuously<\/strong> to the packed chromatography column at a consistent <strong>pulsation-free reproducible flow rate<\/strong>.<\/p>\n<p>The sample mixture loaded on top of the chromatographic column is separated by passing through the solid stationary phase (resin):<\/p>\n<p>The constituents of the fermentation broth are adsorbed and desorbed at a different extent on the stationary phase, whereas\u00a0<strong>the components in the mobile phase slowly <\/strong><strong>separate<\/strong> from one another.<\/p>\n<p>As the mobile phase continues to flow through the column, the column effluent or eluate,\u00a0are\u00a0<strong>collected in fractions<\/strong>\u00a0at the lower end of the chromatographic column\u00a0<strong>with the help of the OMNICOLL fraction collector<\/strong>\u00a0.<\/p>\n<p>A detector to continuously monitor protein elution from the column or the\u00a0<strong>analysis of the fractions<\/strong>\u00a0gives information\u00a0about the <strong>concentration of the desired protein<\/strong> in fraction\u00a0for further processing, for example: by gel electrophoresis.<\/p>\n<p>\u00a0<\/p>\n<hr \/>\n<\/div><\/div><\/div><\/div><div id=\"pg-1707-4\"  class=\"panel-grid panel-no-style\"  style=\"margin-bottom: 50px\" ><div id=\"pgc-1707-4-0\"  class=\"panel-grid-cell\" ><div id=\"panel-1707-4-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"4\" ><div class=\"textwidget\"><h2 style=\"text-align: center;\">Optimization of elution methods for protein purification<\/h2>\n<p>Choosing the right elution buffer, in liquid chromatography for enzyme purification, can considerably improve the quality of the separation and the analysis time.<\/p>\n<\/div><\/div><\/div><\/div><div id=\"pg-1707-5\"  class=\"panel-grid panel-has-style\"  style=\"margin-bottom: 50px\" ><div class=\"siteorigin-panels-stretch panel-row-style-full-width panel-row-style panel-row-style-for-1707-5\" style=\"background-color: #f7f7f7; \" data-stretch-type=\"full\" ><div id=\"pgc-1707-5-0\"  class=\"panel-grid-cell\" ><div id=\"panel-1707-5-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"5\" ><div style=\"padding: 25px 25px 25px 25px;\" class=\"panel-widget-style panel-widget-style-for-1707-5-0-0\" ><div class=\"textwidget\"><h3>Isocratic elution in liquid column chromatography<\/h3>\n<p>In liquid chromatography, the proteins are separated under <strong>constant solvent composition<\/strong> (i.e. isocratic elution):<\/p>\n<ul>\n<li>When samples are not very complex and also, when the sample components do not have similar retention times.<\/li>\n<\/ul>\n<p>Besides, for the most tightly adsorbed proteins, the elution with constant mobile-phase composition will <b>take very long time to be eluted<\/b>.<\/p>\n<p>Buffer and peristaltic pump are used for isocratic elution.<\/p>\n<\/div><\/div><\/div><\/div><div id=\"pgc-1707-5-1\"  class=\"panel-grid-cell\" ><div id=\"panel-1707-5-1-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"6\" ><div style=\"padding: 25px 25px 25px 25px;\" class=\"panel-widget-style panel-widget-style-for-1707-5-1-0\" ><div class=\"textwidget\"><h3>Gradient elution - for purification of proteins from complex mixtures (biological samples)<\/h3>\n<p>In gradient elution, the composition of the <strong>mobile phase changes<\/strong> during the separation process and influences the retention of analytes.<\/p>\n<p>The gradient elution is advantageously used in protein purification:<\/p>\n<ul>\n<li><strong>Complex biological samples<\/strong><\/li>\n<li><strong>M<\/strong><b>ixtures with known properties<\/b><\/li>\n<li><strong>C<\/strong><b>omponents with similar retention times<\/b><\/li>\n<\/ul>\n<p>If gradient elution is used in liquid column chromatography for the purification of proteins, then\u00a0<strong>the retention time can be shortened.<\/strong><\/p>\n<p><span data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.4.0.4\">Gradient elution is carried out\u00a0with\u00a0<\/span><span data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.4.0.5\"><strong>two buffers<\/strong> and <strong>one or two peristaltic pumps.<\/strong><\/span><\/p>\n<\/div><\/div><\/div><\/div><\/div><\/div><div id=\"pg-1707-6\"  class=\"panel-grid panel-no-style\"  style=\"margin-bottom: 50px\" ><div id=\"pgc-1707-6-0\"  class=\"panel-grid-cell\" ><div id=\"panel-1707-6-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"7\" ><div class=\"textwidget\"><h3 style=\"text-align: center;\">Gradient elution and elution profile<\/h3>\n<p>Gradient elution implies that the mobile-phase composition will be varied during sample separation for optimum separation in minimum time.<\/p>\n<p>With the different composition of the eluent (gradient elution), the retention factor and thus, the capacity factor could be influenced.<\/p>\n<h4>The gradient profiles:<\/h4>\n<p style=\"padding-left: 90px;\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-1939\" src=\"https:\/\/www.fractioncollector.info\/wp-content\/uploads\/Linear-gradient-elution-of-proteins.jpg\" alt=\"\" width=\"238\" height=\"212\" \/> <img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-1940\" src=\"https:\/\/www.fractioncollector.info\/wp-content\/uploads\/Segmented-gradient-elution-of-proteins.jpg\" alt=\"\" width=\"238\" height=\"212\" \/>\u00a0<img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-1947 size-full\" src=\"https:\/\/www.fractioncollector.info\/wp-content\/uploads\/Stepwise-gradient-elution-of-proteins.jpg\" alt=\"\" width=\"238\" height=\"212\" srcset=\"https:\/\/www.fractioncollector.info\/wp-content\/uploads\/Stepwise-gradient-elution-of-proteins.jpg 238w, https:\/\/www.fractioncollector.info\/wp-content\/uploads\/Stepwise-gradient-elution-of-proteins-150x134.jpg 150w\" sizes=\"auto, (max-width: 238px) 100vw, 238px\" \/><\/p>\n<p>Linear gradients are generally preferred; it can be implemented in <strong>linear, segmented, convex, and concave shapes<\/strong>. The non-linear gradients offer several distinct advantages, including reduced separation time, improved sample resolution, and higher detection sensitivity.<\/p>\n<\/div><\/div><\/div><\/div><div id=\"pg-1707-7\"  class=\"panel-grid panel-has-style\"  style=\"margin-bottom: 50px\" ><div class=\"siteorigin-panels-stretch panel-row-style-full-width panel-row-style panel-row-style-for-1707-7\" data-stretch-type=\"full\" ><div id=\"pgc-1707-7-0\"  class=\"panel-grid-cell\" ><div id=\"panel-1707-7-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"8\" ><div class=\"textwidget\"><p style=\"text-align: left;\"><a href=\"https:\/\/www.lambda-instruments.com\/peristaltic-pumps\/\" target=\"_blank\" rel=\"noopener\"><img loading=\"lazy\" decoding=\"async\" class=\"alignleft wp-image-1952\" src=\"https:\/\/www.fractioncollector.info\/wp-content\/uploads\/Compact-and-reliable-LAMBDA-lab-peristaltic-pumps.gif\" alt=\"\" width=\"493\" height=\"358\" \/><\/a><\/p>\n<\/div><\/div><\/div><div id=\"pgc-1707-7-1\"  class=\"panel-grid-cell\" ><div class=\"panel-cell-style panel-cell-style-for-1707-7-1\" ><div id=\"panel-1707-7-1-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"9\" ><div style=\"padding: 25px 25px 25px 25px;\" class=\"panel-widget-style panel-widget-style-for-1707-7-1-0\" ><div class=\"textwidget\"><p style=\"text-align: left;\">\n<\/p><p style=\"text-align: left;\"><em>Biochemists recommend <strong>LAMBDA peristaltic liquid pumps<\/strong> which guarantee pulsation-free application of the mobile phase : <a href=\"https:\/\/www.lambda-instruments.com\/peristaltic-pumps\/\" target=\"_blank\" rel=\"noopener\">www.lambda-instruments.com\/peristaltic-pumps\/<\/a>.<br \/>\n<\/em><\/p>\n<\/div><\/div><\/div><\/div><\/div><\/div><\/div><div id=\"pg-1707-8\"  class=\"panel-grid panel-no-style\"  style=\"margin-bottom: 50px\" ><div id=\"pgc-1707-8-0\"  class=\"panel-grid-cell\" ><div id=\"panel-1707-8-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"10\" ><div class=\"textwidget\"><h3 style=\"text-align: center;\">Gradient generation with reliable peristaltic pumps<\/h3>\n<p><span data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.0.0.0\">LAMBDA lab peristaltic pumps are programmable and remote controllable to achieve\u00a0<\/span><strong>linear, stepwise and segmented gradient elution<\/strong><span data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.0.0.2\">.<\/span><\/p>\n<\/div><\/div><\/div><\/div><div id=\"pg-1707-9\"  class=\"panel-grid panel-no-style\"  style=\"margin-bottom: 50px\" ><div id=\"pgc-1707-9-0\"  class=\"panel-grid-cell\" ><div id=\"panel-1707-9-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"11\" ><div style=\"padding: 25px 25px 25px 25px;\" class=\"panel-widget-style panel-widget-style-for-1707-9-0-0\" ><div class=\"textwidget\"><h3 class=\"font_4\" data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.d\"><span data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.d.0\">Gradient elution with one pump<\/span><\/h3>\n<p class=\"font_8\" data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.f\"><span data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.f.0\">Gradient elution can also be carried out with one peristaltic pump: the containers with\u00a0<strong>elution buffer A &amp; B<\/strong> are <strong>connected by a siphon<\/strong>\u00a0or tube at the bottom with a valve to adjust the flow<\/span><\/p>\n<ol class=\"font_8\" data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.g\">\n<li data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.g.0\">\n<p class=\"font_8\" data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.g.0.0\"><span data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.g.0.0.0\">Elution buffer A\u00a0is consistently drawn into the well-stirred beaker with\u00a0elution buffer B, where\u00a0the\u00a0<strong>buffer concentration\u00a0being altered<\/strong><\/span><\/p>\n<\/li>\n<li data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.g.1\">\n<p class=\"font_8\" data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.g.1.0\"><span data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.g.1.0.0\">Programmable LAMBDA peristaltic pump used to\u00a0<strong>pump the\u00a0gradient mixture<\/strong>\u00a0into the chromatography column<\/span><\/p>\n<\/li>\n<li data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.g.2\">\n<p class=\"font_8\" data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.g.2.0\"><span data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.g.2.0.0\">The eluate from the column passed through the\u00a0detector\u00a0to measure\u00a0pH, Absorbance 280 nm &amp; conductance<\/span><\/p>\n<\/li>\n<li data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.g.3\">\n<p class=\"font_8\" data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.g.3.0\"><span data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.g.3.0.0\">From the detector, the\u00a0<strong>eluate fractionation\u00a0collected<\/strong>\u00a0by the\u00a0OMNICOLL fraction collector\u00a0into the desired containers\u00a0<em>(e.g.: bottles, beakers, tubes, vials, etc.)<\/em><\/span><\/p>\n<\/li>\n<\/ol>\n<\/div><\/div><\/div><\/div><div id=\"pgc-1707-9-1\"  class=\"panel-grid-cell\" ><div id=\"panel-1707-9-1-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"12\" ><div style=\"padding: 25px 25px 25px 25px;\" class=\"panel-widget-style panel-widget-style-for-1707-9-1-0\" ><div class=\"textwidget\"><h3 class=\"font_4\" data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.8\"><span data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.8.0\">Gradient elution with two pumps<\/span><\/h3>\n<p class=\"font_8\" data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.a\"><span data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.a.0\"><strong>Two programmable LAMBDA peristaltic\u00a0pumps<\/strong>\u00a0are used\u00a0for reproducible mixing of the eluents for\u00a0gradient\u00a0elution.<\/span><\/p>\n<ol class=\"font_8\" data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.b\">\n<li data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.b.0\">\n<p class=\"font_8\" data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.b.0.0\"><span data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.b.0.0.0\">The <strong>change in the composition over time<\/strong> can be\u00a0<strong>programmed<\/strong> directly on the LAMBDA peristaltic pump display or it can be\u00a0recorded and displayed as a real-time graph\u00a0using the\u00a0PNet PC control\u00a0software.<\/span><\/p>\n<\/li>\n<li data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.b.2\">\n<p class=\"font_8\" data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.b.2.0\"><span data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.b.2.0.0\">The\u00a0<strong>gradient mixture passes through the chromatography column<\/strong>\u00a0and the components are separated.<\/span><\/p>\n<\/li>\n<li data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.b.3\">\n<p class=\"font_8\" data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.b.3.0\"><span data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.b.3.0.0\">The eluate from the column passed through the detector to measure the\u00a0<strong>pH, absorption 280 nm and conductance<\/strong>.<\/span><\/p>\n<\/li>\n<li data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.b.4\">\n<p class=\"font_8\" data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.b.4.0\"><span data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.b.4.0.0\">Automated fraction collector LAMBDA OMNICOLL,\u00a0<strong>fractionates the eluate<\/strong>\u00a0and collects the fractions based on the programmed time or drop counter.<\/span><\/p>\n<\/li>\n<\/ol>\n<\/div><\/div><\/div><\/div><\/div><div id=\"pg-1707-10\"  class=\"panel-grid panel-no-style\"  style=\"margin-bottom: 50px\" ><div id=\"pgc-1707-10-0\"  class=\"panel-grid-cell\" ><div id=\"panel-1707-10-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child\" data-index=\"13\" style=\"margin-bottom: 50px\" ><div class=\"textwidget\"><hr \/>\n<p>\u00a0<\/p>\n<\/div><\/div><div id=\"panel-1707-10-0-1\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-last-child\" data-index=\"14\" ><div class=\"textwidget\"><h2 class=\"font_3\" style=\"text-align: center;\" data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.i\"><span data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.i.0\">Requirements of the pumping system for column chromatography<\/span><\/h2>\n<\/div><\/div><\/div><\/div><div id=\"pg-1707-11\"  class=\"panel-grid panel-no-style\"  style=\"margin-bottom: 50px\" ><div id=\"pgc-1707-11-0\"  class=\"panel-grid-cell\" ><div id=\"panel-1707-11-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"15\" ><div class=\"textwidget\"><p class=\"font_8\" data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.k\"><span data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.k.0\">The most important requirements of the pumping system for column chromatography are:<\/span><\/p>\n<ul class=\"font_8\" data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.l\">\n<li data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.l.0\">\n<p class=\"font_8\" data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.l.0.0\"><span data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.l.0.0.0\"><strong>Constant and reproducible flow rate<\/strong><br data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.l.0.0.0.1\" \/>The reproducibility depends on the retention times of the peaks. The retention times are dependent on the flow rate.<\/span><\/p>\n<\/li>\n<li data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.l.1\">\n<p class=\"font_8\" data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.l.1.0\"><span data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.l.1.0.0\"><strong>Pulsation-free flow<\/strong><br data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.l.1.0.0.1\" \/>The stationary phase has to be protected against pressure surges. The baseline of the detector should remain stable.<\/span><\/p>\n<\/li>\n<\/ul>\n<p>\u00a0<\/p>\n<p>LAMBDA MULTIFLOW programmable peristaltic pumps, which come with a 5 years warranty, are recommended to achieve reproducible flow rates.<\/p>\n<\/div><\/div><\/div><div id=\"pgc-1707-11-1\"  class=\"panel-grid-cell\" ><div id=\"panel-1707-11-1-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"16\" ><div class=\"textwidget\"><p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-1959\" src=\"https:\/\/www.fractioncollector.info\/wp-content\/uploads\/Peristaltic_pumps_LAMBDA.jpg\" alt=\"LAMBDA programmable laboratory peristaltic pumps\" width=\"394\" height=\"328\" srcset=\"https:\/\/www.fractioncollector.info\/wp-content\/uploads\/Peristaltic_pumps_LAMBDA.jpg 394w, https:\/\/www.fractioncollector.info\/wp-content\/uploads\/Peristaltic_pumps_LAMBDA-150x125.jpg 150w, https:\/\/www.fractioncollector.info\/wp-content\/uploads\/Peristaltic_pumps_LAMBDA-334x278.jpg 334w\" sizes=\"auto, (max-width: 394px) 100vw, 394px\" \/><\/p>\n<\/div><\/div><\/div><\/div><div id=\"pg-1707-12\"  class=\"panel-grid panel-no-style\"  style=\"margin-bottom: 50px\" ><div id=\"pgc-1707-12-0\"  class=\"panel-grid-cell\" ><div id=\"panel-1707-12-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"17\" ><div class=\"textwidget\"><h2 class=\"font_3\" style=\"text-align: center;\" data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.i\"><span data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.i.0\">Requirements of the fraction for protein purification<\/span><\/h2>\n<\/div><\/div><\/div><\/div><div id=\"pg-1707-13\"  class=\"panel-grid panel-no-style\"  style=\"margin-bottom: 50px\" ><div id=\"pgc-1707-13-0\"  class=\"panel-grid-cell\" ><div id=\"panel-1707-13-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"18\" ><div class=\"textwidget\"><p class=\"font_8\" data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.k\"><span data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.k.0\">The most important requirements of the fraction collector for liquid column chromatography are:<\/span><\/p>\n<ul class=\"font_8\" data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.l\">\n<li data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.l.0\">\n<p class=\"font_8\" data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.l.0.0\"><span data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.l.0.0.0\"><strong>Unrestricted capacity<\/strong><br data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.l.0.0.0.1\" \/>The possibility to collect unlimited number of fractions is extremely important, especially during process optimization.<\/span><\/p>\n<\/li>\n<li data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.l.1\">\n<p class=\"font_8\" data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.l.1.0\"><span data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.l.1.0.0\"><strong>Different volume range<\/strong><br data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.l.1.0.0.1\" \/>To be able to adapt the fraction collector for various laboratory experiments and pilot scale processes.<\/span><\/p>\n<\/li>\n<\/ul>\n<p>\u00a0<\/p>\n<p>The customizable fraction collector OMNICOLL\u00a0is ideal for efficient process optimization in the laboratory and pilot scale.<\/p>\n<p>\u00a0<\/p>\n<\/div><\/div><\/div><div id=\"pgc-1707-13-1\"  class=\"panel-grid-cell\" ><div id=\"panel-1707-13-1-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"19\" ><div class=\"textwidget\"><p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-928 size-full\" src=\"https:\/\/www.fractioncollector.info\/wp-content\/uploads\/figure3.8-1-Example-of-taking-4x2-fractions-in-a-4-channel-configuration.jpg\" alt=\"\" width=\"665\" height=\"684\" srcset=\"https:\/\/www.fractioncollector.info\/wp-content\/uploads\/figure3.8-1-Example-of-taking-4x2-fractions-in-a-4-channel-configuration.jpg 665w, https:\/\/www.fractioncollector.info\/wp-content\/uploads\/figure3.8-1-Example-of-taking-4x2-fractions-in-a-4-channel-configuration-146x150.jpg 146w, https:\/\/www.fractioncollector.info\/wp-content\/uploads\/figure3.8-1-Example-of-taking-4x2-fractions-in-a-4-channel-configuration-270x278.jpg 270w\" sizes=\"auto, (max-width: 665px) 100vw, 665px\" \/><\/p>\n<\/div><\/div><\/div><\/div><\/div>","protected":false},"excerpt":{"rendered":"<p>Protein purification: Liquid chromatography &amp; gradient elution with OMNICOLL fraction collector and LAMBDA peristaltic pumps For protein purification and isolation of peptides, nucleic acids or small molecules from the crude mixture of fermentation broth, liquid\u00a0column chromatography (LC) is widely used\u00a0with ion exchange, hydrophobic interaction, affinity or size exclusion in downstream processing (DSP).In order to optimize [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":2635,"comment_status":"closed","ping_status":"closed","sticky":true,"template":"","format":"standard","meta":{"footnotes":""},"categories":[132,4],"tags":[131,126,128,127,14,125,130,129],"class_list":["post-1707","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-post-name","category-omnicoll","tag-automatic-fraction-collector","tag-gradient-elution","tag-linear-gradient-elution","tag-liquid-chromatography","tag-peristaltic-pump","tag-protein-purification","tag-segmented-stepwise-gradient-elution","tag-stepwise-gradient-elution","post-with-thumbnail","post-with-thumbnail-icon"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.6 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Successful Protein Purification: Gradient elution &#8211; LAMBDA OMNICOLL | LAMBDA Laboratory Instruments<\/title>\n<meta name=\"description\" content=\"Important 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