{"id":1794,"date":"2017-04-04T13:42:00","date_gmt":"2017-04-04T11:42:00","guid":{"rendered":"https:\/\/www.fractioncollector.info\/?p=1794"},"modified":"2024-06-27T17:55:49","modified_gmt":"2024-06-27T15:55:49","slug":"perifusion-der-inselzellen-mit-automatisiertem-fraktionssammler-omnicoll","status":"publish","type":"post","link":"https:\/\/www.fractioncollector.info\/de\/islets-perifusion-with-automated-fraction-collector-omnicoll\/","title":{"rendered":"Perifusion der Inselzellen mit automatisiertem Fraktionssammler"},"content":{"rendered":"

Inseldurchblutung: Automatisierte Mehrkanal-Fraktionssammlung mit LAMBDA OMNICOLL<\/span><\/strong><\/h1>\n<\/div><\/div><\/div><\/div><\/div>
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\n\t\t\t\t\t\tPankreasinseln\t\t\t\t\t\t<\/h2>\n\t\t\t\t\t\t<\/div>\n<\/div><\/div><\/div>
\n
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Langerhans-Inseln<\/strong> spielen vermutlich eine Schl\u00fcsselrolle in der Pathophysiologie von Typ-1- und Typ-2-Diabetes<\/strong> aufgrund der Unf\u00e4higkeit der Insel-\u03b2-Zellen, ausreichend Insulin zur Regulierung des Blutzuckerspiegels auszusch\u00fctten.<\/p>\n

Das endg\u00fcltige Ma\u00df f\u00fcr die Qualit\u00e4t der \u03b2-Zellen in einer Insel ist die Messung der \u03b2-Zellfunktion, d. h. die F\u00e4higkeit der Inseln, Insulin kontrolliert als Reaktion auf kleinste Ver\u00e4nderungen des umgebenden Glukosespiegels freizusetzen.<\/p>\n

Die Nutzung von Perifusionssystemen zum Verst\u00e4ndnis der \u03b2-Zellkinetik in vitro war ein Schl\u00fcsselfaktor f\u00fcr die Aufkl\u00e4rung der Physiologie und Pathophysiologie der Inselzellen. Die Inselzell-Perifusion umfasst die Exposition der Inselzellen gegen\u00fcber einem kontinuierlichen Fluss von N\u00e4hrstoffen, Gas oder Reagenzien<\/strong>. Mehrere Forscher ver\u00f6ffentlichten Perifusionssysteme mit Kontrolle \u00fcber Temperatur, pH-Wert und Durchflussrate des Perifusats.<\/p>\n

Derzeit gibt es kein standardisiertes, kosteng\u00fcnstiges Ger\u00e4t und kein Protokoll, das sich in jedem Labor problemlos reproduzieren l\u00e4sst.<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div>

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\n\t\t\t\t\t\tINSELN PERIFUSION\t\t\t\t\t\t<\/h2>\n\t\t\t\t\t\t<\/div>\n<\/div><\/div><\/div>
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Der Einrichtung zur Inselzell-Perifusion<\/strong> besteht aus vier Perifusionss\u00e4ulen<\/strong> angeschlossen an vier Kanal-Isamtec-Pumpen, um gleichzeitig vier verschiedene Perifusionsl\u00f6sungen in vier parallele, Inselzellen enthaltende S\u00e4ulen zu pumpen. Durchflussrate von 100 \u00b5L\/min<\/strong>. Die kontinuierliche oder dynamische Perfusion der L\u00f6sung, die Glucose und Sekretagoga enth\u00e4lt, durch die Inselzellen ist die genaueste Methode zur Beurteilung der regulierten Insulinfreisetzung in vitro.<\/p>\n

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Mit dem automatisierten Mehrkanal-Fraktionssammler LAMBDA OMNICOLL k\u00f6nnen beliebige Peristaltikpumpen von Drittanbietern mit analogen Signalen verwendet werden.\u00a0<\/em><\/p>\n<\/blockquote>\n

\"Perifusion<\/p>\n

Die S\u00e4ulen, die die Inselzellen und das Perifusionsmedium enthielten, wurden bei 37 \u00b0C gehalten. in einer temperaturkontrollierten Kammer.<\/strong> Die Inseln wurden pro Stimulus 10 \u2013 15 min lang perfundiert, um eine ausreichende Exposition der Inseln und die Sammlung ihrer Sekretionsprodukte zu erm\u00f6glichen.<\/p>\n

Perifusate (100 \u03bcL\/min) aus 4 S\u00e4ulen wurden gleichzeitig jede Minute mit einem automatisierten System aufgefangen. 4-Kanal-Fraktionssammler LAMBDA OMNICOLL in 96-Well-Platten<\/strong>. Der LAMBDA OMNICOLL 4-Kanal-Probensammler w\u00e4hlt jede Minute die obersten 4 Vertiefungen der 96-Well-Platte aus. (A, B, C und D \u2013 jede dieser Bezeichnungen steht f\u00fcr eine andere Behandlungsbedingung)<\/em> Sammle 12 Minuten lang Br\u00fcche und gehe dann zu den unteren vier Zeilen \u00fcber. (Zeilen E, F, G und H)<\/em> der 96-Well-Platte.<\/p>\n

Das Perifusat in den Sammelplatten mit 96 Vertiefungen wurde bei <4 \u00b0C gehalten, um die Integrit\u00e4t der Analyten zu erhalten.<\/p>\n

\n

Die Mehrkanal-Fraktionssammlerbasen von LAMBDA OMNICOLL (die die Perifusatproben enthalten) k\u00f6nnen in ein Kaltbad oder einen anderen temperaturstabilisierten Beh\u00e4lter gestellt werden.<\/em><\/p>\n<\/blockquote>\n

Die gesammelten Proben des Perifusates wurden untersucht auf Insulin-, Glucagon- oder Somatostatinkonzentration<\/strong>. Die Messung der verschiedenen Hormone kann in verschiedenen Vertiefungen derselben 96-Well-Platte erfolgen.
\nPrinzipiell 96 Proben von Pr\u00e4parationen mehrerer Inseln<\/strong> Durch die gleichzeitige Messung verschiedener experimenteller Bedingungen wird ein Hochdurchsatz-Screening erm\u00f6glicht.<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div>

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Automatisierte Mehrkanal-Fraktionssammlung mit LAMBDA OMNICOLL, konfiguriert f\u00fcr 96-Well-Platten<\/h2>\n

F\u00fcr 96-Well-Platten, weiter automatisierte Mehrkanal-LAMBDA-OMNICOLL-Fraktionssammler<\/a> wurde angepasst:<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div>

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\n\t

\"Kundenspezifischer<\/p>\n

\n<\/div>\n<\/div><\/div><\/div>

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NEU! Konfigurationsversion 2022<\/strong><\/a>
\nBeispiel:<\/em> Kundenspezifischer 6-Kanal-Fraktionssammler LAMBDA OMNICOLL f\u00fcr 96-Well-Platten.<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div>

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\n\t

Weitere Beispiele: \"Fraktionssammlung<\/p>\n

Bruchsammlung mit 4 Kan\u00e4le<\/strong> in 96-Well-Platten zur Inselperfusion<\/p>\n<\/div>\n<\/div><\/div><\/div>

\n
\n\t

\"Automatisierter<\/p>\n

Automatisierter Mehrkanal-Fraktionssammler f\u00fcr 6 simultane Datenstr\u00f6me<\/strong> (1x6) in 96-Well-Platten<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div>

\n
\n\t

\"Kundenspezifischer<\/p>\n

Kundenspezifischer LAMBDA OMNICOLL Mehrkanal-Autosampler mit 2 gleichzeitige Str\u00f6me<\/strong> und zwei LAMBDA-Pumpen<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div><\/div>","protected":false},"excerpt":{"rendered":"

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