{"id":2677,"date":"2021-05-13T15:48:20","date_gmt":"2021-05-13T13:48:20","guid":{"rendered":"https:\/\/www.fractioncollector.info\/?p=2677"},"modified":"2023-07-26T07:51:12","modified_gmt":"2023-07-26T05:51:12","slug":"perfuzni-system-pro-pankreaticke-ostruvky","status":"publish","type":"post","link":"https:\/\/www.fractioncollector.info\/de\/perfuzni-system-pro-pankreaticke-ostruvky\/","title":{"rendered":"Perf\u00fazn\u00ed syst\u00e9m pro pankreatick\u00e9 ostr\u016fvky"},"content":{"rendered":"

Perf\u00faze ostr\u016fvk\u016f s automatick\u00fdm v\u00edcekan\u00e1lov\u00fdm sb\u011bra\u010dem frakc\u00ed OMNICOLL<\/span><\/strong><\/h1>\n<\/div><\/div><\/div><\/div><\/div>
\n\t\t\t\t\t\t\t

\n\t\t\t\t\t\tPANKREATICK\u00c9 OSTR\u016eVKY\t\t\t\t\t\t<\/h1>\n\t\t\t\t\t\t<\/div>\n<\/div><\/div><\/div>
\n
\n\t

P\u0159edpokl\u00e1d\u00e1 se, \u017ee pankreatick\u00e9 ostr\u016fvky hraj\u00ed kl\u00ed\u010dovou roli v patofyziologii diabetu 1. typu<\/strong> a 2. typu<\/strong>, kdy \u03b2-bu\u0148ky v Langerhansov\u00fdch ostr\u016fvc\u00edch nevylu\u010duj\u00ed dostate\u010dn\u00e9 mno\u017estv\u00ed inzul\u00ednu k regulaci gluk\u00f3zy v krvi.<\/p>\n

\u03b2-bu\u0148ky pankreatu uvol\u0148uj\u00ed insulin v reakci na zv\u00fd\u0161enou plazmatickou koncentraci gluk\u00f3zy. Sekreci insulinu zvy\u0161uj\u00ed i jin\u00e9 l\u00e1tky nap\u0159\u00edklad arginin a leucin.<\/p>\n

Pou\u017eit\u00ed perf\u00fazn\u00edch syst\u00e9m\u016f je kl\u00ed\u010dov\u00fdm faktorem p\u0159i objasn\u011bn\u00ed fyziologie a patofyziologie pankreatick\u00fdch ostr\u016fvk\u016f a k pochopen\u00ed kinetiky \u03b2-bun\u011bk in vitro. Perf\u00faze ostr\u016fvk\u016f zahrnuje vystaven\u00ed ostr\u016fvk\u016f nep\u0159etr\u017eit\u00e9mu toku \u017eivin, plynu nebo \u010dinidel. Byli publikov\u00e1ny perf\u00fazn\u00ed syst\u00e9my s kontrolou teploty, pH a rychlosti toku perifus\u00e1tu bun\u011bk.<\/p>\n

V sou\u010dasn\u00e9 dob\u011b neexistuje \u017e\u00e1dn\u00fd standardizovan\u00fd a levn\u00fd p\u0159\u00edstroj a protokol, kter\u00fd by se dal snadno replikovat v jak\u00e9koli laborato\u0159i.<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div>

\n\t\t\t\t\t\t\t

\n\t\t\t\t\t\tPERF\u00daZE OST\u016eVK\u016e\t\t\t\t\t\t<\/h1>\n\t\t\t\t\t\t<\/div>\n<\/div><\/div><\/div>
\n
\n\t

Sestava pro perf\u00fazi pankreatick\u00fdch ostr\u016fvk\u016f se skl\u00e1d\u00e1 ze \u010dty\u0159 kolon<\/strong> spojen\u00fdch s 4kan\u00e1lovou peristaltickou pumpou ISMATEC, kter\u00e1 umo\u017e\u0148uje sou\u010dasnou cirkulaci 4 r\u016fzn\u00fdch perf\u00fazn\u00edch roztok\u016f do 4 paraleln\u00edch kolon s ostr\u016fvky s pr\u016ftokem 100 \u00b5L\/min. <\/strong>Kontinu\u00e1ln\u00ed pr\u016ftok \u010di dynamick\u00e1 perf\u00faze roztoku obsahuj\u00edc\u00edho gluk\u00f3zu a sekretagogy p\u0159es ostr\u016fvky umo\u017e\u0148uje nejp\u0159esn\u011bj\u0161\u00ed hodnocen\u00ed regulovan\u00e9ho uvol\u0148ov\u00e1n\u00ed inzul\u00ednu in vitro.<\/p>\n

\n

Dal\u0161\u00ed mo\u017en\u00e9 konfigurace pro v\u00edcekanalov\u00fd sb\u011bra\u010d frakc\u00ed OMNICOLL s 96jamkov\u00fdmi desti\u010dkami (nebo jin\u00fdmi n\u00e1dobami).<\/em><\/p>\n<\/blockquote>\n<\/div>\n<\/div><\/div>

\n
\n\t\t\"Islets\n\t<\/div>\n\n<\/div><\/div>
\n
\n\t

N\u00e1doba se vzorky, kter\u00e1 obsahuje ostr\u016fvky a perf\u00fazn\u00ed roztoky, byla udr\u017eov\u00e1na p\u0159i 37 \u00b0 C \u00a0ve vestav\u011bn\u00e9 komo\u0159e s \u0159\u00edzenou teplotou. Ostr\u016fvky byly stimulov\u00e1ny po dobu 10\u201315 minut na jeden stimul, co\u017e umo\u017enilo jejich expozici a n\u00e1sledn\u00fd sb\u011br jejich vzork\u016f.<\/p>\n

Perfuz\u00e1t byl sou\u010dasn\u011b sb\u00edr\u00e1n ka\u017edou minutu ze \u010dty\u0159 v\u00fdtokov\u00fdch kolon pomoc\u00ed automatick\u00e9ho sb\u011bra\u010de frakc\u00ed do 96jamkov\u00fdch desti\u010dek. \u00a0\u010cty\u0159kan\u00e1lov\u00fd automatick\u00fd sb\u011bra\u010d OMNICOLL sb\u00edr\u00e1 horn\u00ed 4 jamky 96jamkov\u00e9 desti\u010dky ka\u017edou minutu (A, B, C a D - ka\u017ed\u00e1 p\u0159edstavuje odli\u0161n\u00e9 zpracov\u00e1n\u00ed), aby shrom\u00e1\u017edil 12 minut frakc\u00ed a pot\u00e9 se p\u0159esunul do spodn\u00edch \u010dty\u0159 \u0159\u00e1dk\u016f (E , F, G & H \u0159ady) 96jamkov\u00e9 desti\u010dky.<\/p>\n

\n

Dal\u0161\u00ed mo\u017en\u00e9 konfigurace pro v\u00edcekanalov\u00fd sb\u011bra\u010d frakc\u00ed OMNICOLL<\/em><\/a> s 96jamkov\u00fdmi desti\u010dkami (nebo jin\u00fdmi n\u00e1dobami).<\/p>\n<\/blockquote>\n<\/div>\n<\/div><\/div>

\n
\n\t

Perfuz\u00e1t ve sb\u011brn\u00e9 desce byl udr\u017eov\u00e1n na <4 \u00b0 C, aby byla zachov\u00e1na integrita analyt\u016f.<\/p>\n

\n

Z\u00e1kladna sb\u011bra\u010de frakc\u00ed OMNICOLL (obsahuj\u00edc\u00ed frakce\/vzorky) m\u016f\u017ee b\u00fdt um\u00edst\u011bna do studen\u00e9 l\u00e1zn\u011b nebo jin\u00e9 termostabiliza\u010dn\u00ed n\u00e1doby.<\/em><\/p>\n<\/blockquote>\n

Odebran\u00e9 vzorky byly testov\u00e1ny na koncentraci inzul\u00ednu, glukagonu nebo somatostatinu. M\u011b\u0159en\u00ed r\u016fzn\u00fdch hormon\u016f lze prov\u00e1d\u011bt v r\u016fzn\u00fdch jamk\u00e1ch stejn\u00e9 96jamkov\u00e9 desti\u010dky.<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div>

\n
\n\t

DAL\u0160\u00cd V\u00cdCEKAN\u00c1LOV\u00c9 KONFIGURACE S 96JAMKOV\u00ddMI DESTI\u010cKAMI<\/h1>\n<\/div>\n<\/div><\/div><\/div><\/div>
\n
\n\t\"LAMBDA<\/div>\n<\/div><\/div><\/div>
\n
\n\t

LAMBDA OMNICOLL 6kan\u00e1lov\u00fd pro sou\u010dasn\u00fd 6proud\u00fd sb\u011br (1x6) do 96jamkov\u00fdch mikrotitra\u010dn\u00edch desti\u010dek (vodi\u010de hadi\u010dek jsou nastaviteln\u00e9)<\/p>\n<\/div>\n<\/div><\/div><\/div>

\n
\n\t\"Upraven\u00fd<\/div>\n<\/div><\/div><\/div>
\n
\n\t

Upraven\u00fd 4kan\u00e1lov\u00fd sb\u011bra\u010d frakc\u00ed OMNICOLL pro chromatografii ke sb\u011bru 4 proud\u016f do 96jamkov\u00fdch mikrotitra\u010dn\u00edch desti\u010dek (pozice na rameni jsou fixn\u00ed)<\/p>\n<\/div>\n<\/div><\/div><\/div>

\n
\n\t\"Dvoukan\u00e1lov\u00fd<\/div>\n<\/div><\/div>
\n
\n\t

Dvoukan\u00e1lov\u00fd sb\u011bra\u010d frakc\u00ed OMNICOLL se dv\u011bma pumpami LAMBDA pro 96jamkov\u00e9 mikrotitra\u010dn\u00ed desti\u010dky<\/p>\n<\/div>\n<\/div><\/div><\/div>

\n
\n\t

\"Automatick\u00fd<\/p>\n<\/div>\n<\/div><\/div>

\n
\n\t

Automatick\u00fd sb\u011bra\u010d frakc\u00ed OMNICOLL pro chromatografii se sou\u010dasn\u00fdm sb\u011brem 3<\/strong> proud\u016f do 96jamkov\u00e9 mikrotitra\u010dn\u00ed desti\u010dky (vodi\u010de hadi\u010dek jsou nastaviteln\u00e9)<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div><\/div>","protected":false},"excerpt":{"rendered":"

Perf\u00faze ostr\u016fvk\u016f s automatick\u00fdm v\u00edcekan\u00e1lov\u00fdm sb\u011bra\u010dem frakc\u00ed OMNICOLL P\u0159edpokl\u00e1d\u00e1 se, \u017ee pankreatick\u00e9 ostr\u016fvky hraj\u00ed kl\u00ed\u010dovou roli v patofyziologii diabetu 1. typu a 2. typu, kdy \u03b2-bu\u0148ky v Langerhansov\u00fdch ostr\u016fvc\u00edch nevylu\u010duj\u00ed dostate\u010dn\u00e9 mno\u017estv\u00ed inzul\u00ednu k regulaci gluk\u00f3zy v krvi. \u03b2-bu\u0148ky pankreatu uvol\u0148uj\u00ed insulin v reakci na zv\u00fd\u0161enou plazmatickou koncentraci gluk\u00f3zy. Sekreci insulinu zvy\u0161uj\u00ed i jin\u00e9 l\u00e1tky […]<\/p>\n","protected":false},"author":14,"featured_media":1798,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[307],"tags":[],"class_list":["post-2677","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-uncategorized-cs","post-with-thumbnail","post-with-thumbnail-icon"],"yoast_head":"\nPerf\u00fazn\u00ed syst\u00e9m pro pankreatick\u00e9 ostr\u016fvky – LAMBDA OMNICOLL | LAMBDA Laboratory Instruments<\/title>\n<meta name=\"description\" content=\"Perf\u00fazn\u00ed syst\u00e9m pro pankreatick\u00e9 ostr\u016fvky s automatick\u00fdm 4kan\u00e1lov\u00fdm sb\u011bra\u010dem frakc\u00ed OMNICOLL pro sb\u011br perfuz\u00e1tu do 96jamkov\u00e9 desti\u010dky\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" 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