{"id":1794,"date":"2017-04-04T13:42:00","date_gmt":"2017-04-04T11:42:00","guid":{"rendered":"https:\/\/www.fractioncollector.info\/?p=1794"},"modified":"2024-06-27T17:55:49","modified_gmt":"2024-06-27T15:55:49","slug":"perfusion-de-islotes-con-colector-de-fracciones-automatizado-omnicoll","status":"publish","type":"post","link":"https:\/\/www.fractioncollector.info\/es\/islets-perifusion-with-automated-fraction-collector-omnicoll\/","title":{"rendered":"Perfusi\u00f3n de islotes con colector de fracciones automatizado"},"content":{"rendered":"

PERIFUSI\u00d3N DE ISLOTES: Recolecci\u00f3n automatizada de fracciones multicanal con LAMBDA OMNICOLL<\/span><\/strong><\/h1>\n<\/div><\/div><\/div><\/div><\/div>
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\n\t\t\t\t\t\tISLOTES PANCRE\u00c1TICOS\t\t\t\t\t\t<\/h2>\n\t\t\t\t\t\t<\/div>\n<\/div><\/div><\/div>
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Islotes pancre\u00e1ticos<\/strong> Se cree que desempe\u00f1an un papel clave en la fisiopatolog\u00eda de Diabetes tipo 1 y tipo 2<\/strong> debido a que las c\u00e9lulas \u03b2 de los islotes no secretan cantidades suficientes de insulina para regular la glucosa en sangre.<\/p>\n

La medida definitiva de la calidad de las c\u00e9lulas \u03b2 en un islote es la medici\u00f3n de la funci\u00f3n de las c\u00e9lulas \u03b2, es decir, la capacidad de los islotes para liberar insulina de manera controlada en respuesta a cambios m\u00ednimos en los niveles de glucosa ambiental.<\/p>\n

La utilizaci\u00f3n de sistemas de perfusi\u00f3n para comprender la cin\u00e9tica de las c\u00e9lulas \u03b2 in vitro ha sido un factor clave en la elucidaci\u00f3n de la fisiolog\u00eda y fisiopatolog\u00eda de los islotes. La perfusi\u00f3n de los islotes implica la exposici\u00f3n de los islotes a un flujo continuo de nutrientes, gases o reactivos<\/strong>. Varios investigadores han publicado sistemas de perfusi\u00f3n con control de temperatura, pH y caudal del l\u00edquido de perfusi\u00f3n.<\/p>\n

Actualmente no existe ning\u00fan aparato ni protocolo estandarizado y econ\u00f3mico que pueda replicarse f\u00e1cilmente en cualquier laboratorio.<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div>

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\n\t\t\t\t\t\tPERIFUSI\u00d3N DE ISLOTES\t\t\t\t\t\t<\/h2>\n\t\t\t\t\t\t<\/div>\n<\/div><\/div><\/div>
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El Configuraci\u00f3n de la perfusi\u00f3n de los islotes<\/strong> consta de cuatro columnas de perfusi\u00f3n<\/strong> conectado a bombas Isamtec de cuatro canales, para bombear 4 soluciones de perfusi\u00f3n diferentes a 4 columnas paralelas que contienen islotes simult\u00e1neamente con el caudal de 100 \u00b5L\/min<\/strong>. El flujo continuo o la perfusi\u00f3n din\u00e1mica de la soluci\u00f3n que contiene glucosa y secretagogos a trav\u00e9s de los islotes es la evaluaci\u00f3n m\u00e1s precisa de la liberaci\u00f3n regulada de insulina in vitro.<\/p>\n

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Es posible utilizar cualquier bomba perist\u00e1ltica de terceros con se\u00f1ales anal\u00f3gicas mediante el colector de fracciones multicanal automatizado LAMBDA OMNICOLL.\u00a0<\/em><\/p>\n<\/blockquote>\n

\"Perfusi\u00f3n<\/p>\n

Las columnas que conten\u00edan los islotes y el medio de perfusi\u00f3n se mantuvieron a 37 \u00b0C. en una c\u00e1mara con temperatura controlada.<\/strong> Los islotes se perfundieron durante 10-15 minutos por est\u00edmulo para permitir una exposici\u00f3n adecuada de los islotes y la recolecci\u00f3n de su producto de secreci\u00f3n.<\/p>\n

Los perfusados (100 \u03bcL\/min) de 4 columnas se recolectaron simult\u00e1neamente cada minuto mediante un sistema automatizado. Colector de fracciones de 4 canales LAMBDA OMNICOLL en placas de 96 pocillos<\/strong>. El colector de muestras de 4 canales LAMBDA OMNICOLL selecciona los 4 pocillos superiores de la placa de 96 pocillos cada minuto. (A, B, C y D: cada una representa una condici\u00f3n de tratamiento diferente)<\/em> para recopilar 12 minutos de fracciones y luego pasar a las cuatro filas inferiores (Filas E, F, G y H)<\/em> de la placa de 96 pocillos.<\/p>\n

El l\u00edquido de perfusi\u00f3n en las placas de recolecci\u00f3n de 96 pocillos se mantuvo a <4 \u00b0C para preservar la integridad de los analitos.<\/p>\n

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Las bases colectoras de fracciones multicanal LAMBDA OMNICOLL (que contienen las muestras de perfusado) se pueden colocar en un ba\u00f1o fr\u00edo o en cualquier otro recipiente termoestabilizado.<\/em><\/p>\n<\/blockquote>\n

Las muestras recolectadas del perfusado fueron analizadas para concentraci\u00f3n de insulina, glucag\u00f3n o somatostatina<\/strong>. La medici\u00f3n de las diferentes hormonas se puede realizar en diferentes pocillos de la misma placa de 96 pocillos.
\nEn principio, 96 muestras de preparaciones de islotes m\u00faltiples<\/strong> Se pueden realizar mediciones simult\u00e1neas en diferentes condiciones experimentales, lo que permite un cribado de alto rendimiento.<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div>

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Recolecci\u00f3n automatizada de fracciones multicanal con LAMBDA OMNICOLL configurada para placas de 96 pocillos.<\/h2>\n

Para placas de 96 pocillos, adem\u00e1s Colectores de fracciones multicanal automatizados LAMBDA OMNICOLL<\/a> se personaliz\u00f3:<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div>

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\"Colector<\/p>\n

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\u00a1NUEVO! Versi\u00f3n de configuraci\u00f3n 2022<\/strong><\/a>
\nEjemplo:<\/em> Colector de fracciones LAMBDA OMNICOLL de 6 canales personalizado para placas de 96 pocillos.<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div>

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Otros ejemplos: \"Recolecci\u00f3n<\/p>\n

Colecci\u00f3n de fracciones con 4 canales<\/strong> en placas de 96 pocillos para la perfusi\u00f3n de islotes<\/p>\n<\/div>\n<\/div><\/div><\/div>

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\"Colector<\/p>\n

Colector de fracciones multicanal automatizado para 6 transmisiones simult\u00e1neas<\/strong> (1x6) en placas de 96 pocillos<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div>

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\"Colector<\/p>\n

Muestreador autom\u00e1tico multicanal LAMBDA OMNICOLL personalizado con 2 flujos simult\u00e1neos<\/strong> y dos bombas LAMBDA<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div><\/div>","protected":false},"excerpt":{"rendered":"

PERIFUSI\u00d3N DE ISLOTES: Recolecci\u00f3n automatizada de fracciones multicanal con LAMBDA OMNICOLL<\/p>","protected":false},"author":2,"featured_media":1798,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"ai_generated_summary":"","footnotes":""},"categories":[132],"tags":[95,138,139,137,119,140],"class_list":["post-1794","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-post-name","tag-4-channel","tag-96-well-plates","tag-automated-fraction-collector","tag-islets-perifusion","tag-ismatec-multichannel-pump","tag-pancreatic-islets","post-with-thumbnail","post-with-thumbnail-icon"],"yoast_head":"\nIslets perifusion with automated fraction collector – LAMBDA OMNICOLL | LAMBDA Laboratory Instruments<\/title>\n<meta name=\"description\" content=\"Islets perifusion set-up with an automated 4-channel fraction collector OMNICOLL, to collect perifusate into 96-well plates\" \/>\n<meta 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