{"id":1794,"date":"2017-04-04T13:42:00","date_gmt":"2017-04-04T11:42:00","guid":{"rendered":"https:\/\/www.fractioncollector.info\/?p=1794"},"modified":"2024-06-27T17:55:49","modified_gmt":"2024-06-27T15:55:49","slug":"perifusion-des-ilots-avec-collecteur-de-fractions-automatise-omnicoll","status":"publish","type":"post","link":"https:\/\/www.fractioncollector.info\/fr\/islets-perifusion-with-automated-fraction-collector-omnicoll\/","title":{"rendered":"P\u00e9rifusion d'\u00eelots avec collecteur de fractions automatis\u00e9"},"content":{"rendered":"

P\u00c9RIFUSION DES \u00ceLOTS : Collecte automatis\u00e9e de fractions multicanaux avec LAMBDA OMNICOLL<\/span><\/strong><\/h1>\n<\/div><\/div><\/div><\/div><\/div>
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\n\t\t\t\t\t\t\u00ceLES PANCR\u00c9ATIQUES\t\t\t\t\t\t<\/h2>\n\t\t\t\t\t\t<\/div>\n<\/div><\/div><\/div>
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\u00celots pancr\u00e9atiques<\/strong> on pense qu'ils jouent un r\u00f4le cl\u00e9 dans la physiopathologie de Diab\u00e8te de type 1 et de type 2<\/strong> en raison de l'incapacit\u00e9 des cellules \u03b2 des \u00eelots \u00e0 s\u00e9cr\u00e9ter des quantit\u00e9s suffisantes d'insuline pour r\u00e9guler la glyc\u00e9mie.<\/p>\n

La mesure d\u00e9finitive de la qualit\u00e9 des cellules \u03b2 dans un \u00eelot est la mesure de la fonction des cellules \u03b2, c'est-\u00e0-dire la capacit\u00e9 des \u00eelots \u00e0 lib\u00e9rer de l'insuline de mani\u00e8re contr\u00f4l\u00e9e en r\u00e9ponse \u00e0 de minuscules variations des niveaux de glucose ambiant.<\/p>\n

L'utilisation de syst\u00e8mes de p\u00e9rifusion pour comprendre la cin\u00e9tique des cellules \u03b2 in vitro a \u00e9t\u00e9 un facteur cl\u00e9 dans l'\u00e9lucidation de la physiologie et de la physiopathologie des \u00eelots de Langerhans. La p\u00e9rifusion des \u00eelots implique\u2026 exposition des \u00eelots \u00e0 un flux continu de nutriments, de gaz ou de r\u00e9actifs<\/strong>. Plusieurs chercheurs ont publi\u00e9 des syst\u00e8mes de p\u00e9rifusion permettant de contr\u00f4ler la temp\u00e9rature, le pH et le d\u00e9bit du p\u00e9rifusat.<\/p>\n

Il n'existe actuellement aucun appareil ni protocole standardis\u00e9 et peu co\u00fbteux qui puisse \u00eatre facilement reproduit dans n'importe quel laboratoire.<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div>

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\n\t\t\t\t\t\tP\u00c9RIFUSION DES \u00ceLES\t\t\t\t\t\t<\/h2>\n\t\t\t\t\t\t<\/div>\n<\/div><\/div><\/div>
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Le dispositif de p\u00e9rifusion d'\u00eelots<\/strong> se compose de quatre colonnes de p\u00e9rifusion<\/strong> reli\u00e9 \u00e0 quatre pompes Isamtec \u00e0 canaux, pour pomper simultan\u00e9ment 4 solutions de p\u00e9rifusion diff\u00e9rentes dans 4 colonnes parall\u00e8les contenant des \u00eelots. d\u00e9bit de 100 \u00b5L\/min<\/strong>. La perfusion continue ou dynamique de la solution contenant du glucose et des s\u00e9cr\u00e9tagogues \u00e0 travers les \u00eelots constitue l'\u00e9valuation la plus pr\u00e9cise de la lib\u00e9ration r\u00e9gul\u00e9e d'insuline in vitro.<\/p>\n

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Il est possible d'utiliser n'importe quelle pompe p\u00e9ristaltique tierce avec des signaux analogiques gr\u00e2ce au collecteur de fractions multicanaux automatis\u00e9 LAMBDA OMNICOLL.\u00a0<\/em><\/p>\n<\/blockquote>\n

\"Perfusion<\/p>\n

Les colonnes contenant les \u00eelots et le milieu de p\u00e9rifusion ont \u00e9t\u00e9 maintenues \u00e0 37 \u00b0C. dans une chambre \u00e0 temp\u00e9rature contr\u00f4l\u00e9e.<\/strong> Les \u00eelots ont \u00e9t\u00e9 perfus\u00e9s pendant 10 \u00e0 15 minutes par stimulus afin de permettre une exposition ad\u00e9quate des \u00eelots et la collecte de leur produit de s\u00e9cr\u00e9tion.<\/p>\n

Les perfusats (100 \u03bcL\/min) de 4 colonnes ont \u00e9t\u00e9 collect\u00e9s simultan\u00e9ment toutes les minutes par un syst\u00e8me automatis\u00e9. Collecteur de fractions \u00e0 4 canaux LAMBDA OMNICOLL dans des plaques \u00e0 96 puits<\/strong>. Le collecteur d'\u00e9chantillons LAMBDA OMNICOLL \u00e0 4 canaux s\u00e9lectionne les 4 puits sup\u00e9rieurs de la plaque \u00e0 96 puits toutes les minutes. (A, B, C et D - chacun repr\u00e9sentant une condition de traitement diff\u00e9rente)<\/em> pour collecter 12 minutes de fractions puis passer aux quatre rang\u00e9es du bas (Lignes E, F, G et H)<\/em> de la plaque \u00e0 96 puits.<\/p>\n

Le perfusat dans les plaques de collecte \u00e0 96 puits a \u00e9t\u00e9 maintenu \u00e0 <4 \u00b0C pour pr\u00e9server l'int\u00e9grit\u00e9 des analytes.<\/p>\n

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Les bases de collecteurs de fractions multicanaux LAMBDA OMNICOLL (contenant les \u00e9chantillons de perfusat) peuvent \u00eatre plac\u00e9es dans un bain froid ou dans tout autre r\u00e9cipient thermostabilis\u00e9.<\/em><\/p>\n<\/blockquote>\n

Les \u00e9chantillons pr\u00e9lev\u00e9s du liquide de perfusion ont \u00e9t\u00e9 analys\u00e9s pour concentration d'insuline, de glucagon ou de somatostatine<\/strong>. Le dosage des diff\u00e9rentes hormones peut \u00eatre effectu\u00e9 dans diff\u00e9rents puits d'une m\u00eame plaque \u00e0 96 puits.
\nEn principe, 96 \u00e9chantillons provenant de pr\u00e9parations d'\u00eelots multiples<\/strong> Il est possible de mesurer simultan\u00e9ment diff\u00e9rentes conditions exp\u00e9rimentales, ce qui permet un criblage \u00e0 haut d\u00e9bit.<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div>

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Collecte automatis\u00e9e de fractions multicanaux avec LAMBDA OMNICOLL configur\u00e9e pour les plaques \u00e0 96 puits<\/h2>\n

Pour les plaques \u00e0 96 puits, en outre collecteurs de fractions LAMBDA OMNICOLL multicanaux automatis\u00e9s<\/a> personnalis\u00e9 :<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div>

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\"Collecteur<\/p>\n

\n<\/div>\n<\/div><\/div><\/div>

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NOUVEAU ! Configuration Version 2022<\/strong><\/a>
\nExemple:<\/em> Collecteur de fractions LAMBDA OMNICOLL personnalis\u00e9 \u00e0 6 canaux pour plaques 96 puits.<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div>

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Autres exemples : \"Collecte<\/p>\n

Collection de fractions avec 4 canaux<\/strong> dans des plaques \u00e0 96 puits pour la perfusion d'\u00eelots<\/p>\n<\/div>\n<\/div><\/div><\/div>

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\"Collecteur<\/p>\n

Collecteur de fractions multicanal automatis\u00e9 pour 6 flux simultan\u00e9s<\/strong> (1x6) dans des plaques \u00e0 96 puits<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div>

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\"Collecteur<\/p>\n

\u00c9chantillonneur automatique multicanal LAMBDA OMNICOLL personnalis\u00e9 avec 2 flux simultan\u00e9s<\/strong> et deux pompes LAMBDA<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div><\/div>","protected":false},"excerpt":{"rendered":"

P\u00c9RIFUSION DES \u00ceLOTS : Collecte automatis\u00e9e de fractions multicanaux avec LAMBDA OMNICOLL<\/p>","protected":false},"author":2,"featured_media":1798,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"ai_generated_summary":"","footnotes":""},"categories":[132],"tags":[95,138,139,137,119,140],"class_list":["post-1794","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-post-name","tag-4-channel","tag-96-well-plates","tag-automated-fraction-collector","tag-islets-perifusion","tag-ismatec-multichannel-pump","tag-pancreatic-islets","post-with-thumbnail","post-with-thumbnail-icon"],"yoast_head":"\nIslets perifusion with automated fraction collector – LAMBDA OMNICOLL | LAMBDA Laboratory Instruments<\/title>\n<meta name=\"description\" content=\"Islets perifusion set-up with an automated 4-channel fraction collector OMNICOLL, to collect perifusate into 96-well plates\" \/>\n<meta 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