{"id":1707,"date":"2017-01-10T14:44:45","date_gmt":"2017-01-10T12:44:45","guid":{"rendered":"https:\/\/www.fractioncollector.info\/?p=1707"},"modified":"2024-08-05T16:03:04","modified_gmt":"2024-08-05T14:03:04","slug":"cromatografia-de-purificacao-de-proteinas-por-coletor-de-fracoes","status":"publish","type":"post","link":"https:\/\/www.fractioncollector.info\/pt\/fraction-collector-protein-purification-chromatography\/","title":{"rendered":"Purifica\u00e7\u00e3o de prote\u00ednas bem-sucedida: elui\u00e7\u00e3o em gradiente"},"content":{"rendered":"<div id=\"pl-1707\"  class=\"panel-layout\" ><div id=\"pg-1707-0\"  class=\"panel-grid panel-has-style\"  style=\"margin-bottom: 50px\" ><div class=\"siteorigin-panels-stretch panel-row-style-full-width panel-row-style panel-row-style-for-1707-0\" style=\"padding: 0px 0px 0px 0px;\" data-stretch-type=\"full-width-stretch\" ><div id=\"pgc-1707-0-0\"  class=\"panel-grid-cell\" ><div id=\"panel-1707-0-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"0\" ><div class=\"textwidget\"><h1 style=\"text-align: center;\"><span style=\"color: #ffffff; font-size: 35px; text-shadow: 1px 1px #000000;\">Purifica\u00e7\u00e3o de prote\u00ednas: Cromatografia l\u00edquida e elui\u00e7\u00e3o em gradiente<\/span><\/h1>\n<h2 style=\"text-align: center;\"><span style=\"color: #333333; font-size: 20px; text-shadow: 1px 1px #000000;\">com coletor de fra\u00e7\u00f5es OMNICOLL e bombas perist\u00e1lticas LAMBDA<\/span><\/h2>\n<\/div><\/div><\/div><\/div><\/div><div id=\"pg-1707-1\"  class=\"panel-grid panel-no-style\"  style=\"margin-bottom: 50px\" ><div id=\"pgc-1707-1-0\"  class=\"panel-grid-cell\" ><div id=\"panel-1707-1-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"1\" ><div class=\"textwidget\"><p>Para <strong>purifica\u00e7\u00e3o de prote\u00ednas<\/strong> e <strong>isolamento de pept\u00eddeos, \u00e1cidos nucleicos ou pequenas mol\u00e9culas<\/strong> da mistura bruta do caldo de fermenta\u00e7\u00e3o, <strong>l\u00edquido<\/strong>\u00a0<strong>A cromatografia em coluna (LC) \u00e9 amplamente utilizada.<\/strong>\u00a0com troca i\u00f4nica, intera\u00e7\u00e3o hidrof\u00f3bica, afinidade ou exclus\u00e3o por tamanho no processamento a jusante (DSP).<\/p>\n<p>A fim de <strong>otimizar os processos de cromatografia l\u00edquida<\/strong>, O coletor de fra\u00e7\u00f5es OMNICOLL e as confi\u00e1veis bombas perist\u00e1lticas LAMBDA para l\u00edquidos s\u00e3o utilizados com sucesso na pr\u00e1tica laboratorial:<\/p>\n<\/div><\/div><\/div><\/div><div id=\"pg-1707-2\"  class=\"panel-grid panel-no-style\"  style=\"margin-bottom: 50px\" ><div id=\"pgc-1707-2-0\"  class=\"panel-grid-cell\" ><div id=\"panel-1707-2-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"2\" ><div class=\"textwidget\"><h3><span style=\"color: #60351d;\">Exemplo de configura\u00e7\u00e3o de coleta autom\u00e1tica de fra\u00e7\u00f5es<\/span><\/h3>\n<p>Por exemplo, um completo <b>Configura\u00e7\u00e3o de cromatografia em coluna com coleta autom\u00e1tica<\/b> A an\u00e1lise do eluato utilizando o coletor de fra\u00e7\u00f5es OMNICOLL inclui:<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-2635 size-full\" title=\"Coletor de fra\u00e7\u00f5es LAMBDA OMNICOLL e bombas perist\u00e1lticas LAMBDA para purifica\u00e7\u00e3o de prote\u00ednas por elui\u00e7\u00e3o em gradiente de concentra\u00e7\u00e3o.\" src=\"https:\/\/www.fractioncollector.info\/wp-content\/uploads\/OMNICOLL-fraction-collector-LAMBDA-pumps-protein-purification-gradient-elution.jpg\" alt=\"Coletor de fra\u00e7\u00f5es LAMBDA OMNICOLL e bombas perist\u00e1lticas LAMBDA para purifica\u00e7\u00e3o de prote\u00ednas por elui\u00e7\u00e3o em gradiente de concentra\u00e7\u00e3o.\" width=\"700\" height=\"400\" srcset=\"https:\/\/www.fractioncollector.info\/wp-content\/uploads\/OMNICOLL-fraction-collector-LAMBDA-pumps-protein-purification-gradient-elution.jpg 700w, https:\/\/www.fractioncollector.info\/wp-content\/uploads\/OMNICOLL-fraction-collector-LAMBDA-pumps-protein-purification-gradient-elution-418x239.jpg 418w, https:\/\/www.fractioncollector.info\/wp-content\/uploads\/OMNICOLL-fraction-collector-LAMBDA-pumps-protein-purification-gradient-elution-150x86.jpg 150w\" sizes=\"auto, (max-width: 700px) 100vw, 700px\" \/><\/p>\n<ul>\n<li>Dois recipientes semelhantes com <b>Os tamp\u00f5es de elui\u00e7\u00e3o A e B est\u00e3o conectados por um sif\u00e3o.<\/b> (ou tubo na parte inferior com uma v\u00e1lvula para ajustar o fluxo)<\/li>\n<li><b>Tamp\u00e3o de elui\u00e7\u00e3o A<\/b> \u00e9 consistentemente aspirado para dentro do b\u00e9quer com <b>tamp\u00e3o de elui\u00e7\u00e3o B<\/b> que \u00e9 constantemente <b>misturado por agitador magn\u00e9tico<\/b>, deste modo <b>alterando sua concentra\u00e7\u00e3o tamp\u00e3o<\/b><\/li>\n<li>Bomba perist\u00e1ltica LAMBDA program\u00e1vel para <b>bombeie a mistura gradiente<\/b> na coluna<\/li>\n<li><b>Eluato da coluna<\/b> est\u00e1 sendo passado atrav\u00e9s do <b>detector <\/b>(para medir pH, absorb\u00e2ncia a 280 nm, condutividade)<\/li>\n<li>A partir do detector, o <b>fracionamento de prote\u00ednas<\/b> \u00e9 coletado pelo <b>Coletor de fra\u00e7\u00f5es OMNICOLL<\/b> nos recipientes desejados (ex: tubos, frascos, etc.)<\/li>\n<\/ul>\n<p>As prote\u00ednas s\u00e3o elu\u00eddas alterando-se a composi\u00e7\u00e3o do tamp\u00e3o. <i>(for\u00e7a i\u00f4nica, pH, condutividade, concentra\u00e7\u00e3o de sais, etc.)<\/i>. As condi\u00e7\u00f5es de elui\u00e7\u00e3o podem ser alteradas de linear, gradual ou gradual segmentada por <b>alterando o fluxo do tamp\u00e3o A para B<\/b> em que a composi\u00e7\u00e3o da fase m\u00f3vel muda ao longo do tempo.<\/p>\n<\/div><\/div><\/div><\/div><div id=\"pg-1707-3\"  class=\"panel-grid panel-no-style\"  style=\"margin-bottom: 50px\" ><div id=\"pgc-1707-3-0\"  class=\"panel-grid-cell\" ><div id=\"panel-1707-3-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"3\" ><div class=\"textwidget\"><h2 style=\"text-align: center;\">Procedimento<\/h2>\n<p>Na cromatografia em coluna l\u00edquida, \u00e9 importante empacotar a coluna uniformemente.<strong> sem bolhas de ar, canais ou fissuras na fase estacion\u00e1ria<\/strong>.<\/p>\n<p>A bomba perist\u00e1ltica LAMBDA fornece o <strong>fase m\u00f3vel l\u00edquida continuamente<\/strong> para a coluna cromatogr\u00e1fica empacotada de forma consistente <strong>vaz\u00e3o reproduz\u00edvel sem pulsa\u00e7\u00e3o<\/strong>.<\/p>\n<p>A mistura da amostra carregada no topo da coluna cromatogr\u00e1fica \u00e9 separada pela passagem atrav\u00e9s da fase estacion\u00e1ria s\u00f3lida (resina):<\/p>\n<p>Os componentes do caldo de fermenta\u00e7\u00e3o s\u00e3o adsorvidos e dessorvidos em diferentes graus na fase estacion\u00e1ria, enquanto\u00a0<strong>os componentes na fase m\u00f3vel lentamente <\/strong><strong>separar<\/strong> um do outro.<\/p>\n<p>\u00c0 medida que a fase m\u00f3vel continua a fluir pela coluna, o efluente da coluna, ou eluato, s\u00e3o\u00a0<strong>coletados em fra\u00e7\u00f5es<\/strong>\u00a0na extremidade inferior da coluna cromatogr\u00e1fica\u00a0<strong>com a ajuda do coletor de fra\u00e7\u00f5es OMNICOLL<\/strong>\u00a0.<\/p>\n<p>Um detector para monitorar continuamente a elui\u00e7\u00e3o de prote\u00ednas da coluna ou do\u00a0<strong>an\u00e1lise das fra\u00e7\u00f5es<\/strong>\u00a0fornece informa\u00e7\u00f5es sobre o <strong>concentra\u00e7\u00e3o da prote\u00edna desejada<\/strong> em fra\u00e7\u00e3o para processamento posterior, por exemplo: por eletroforese em gel.<\/p>\n<p>\u00a0<\/p>\n<hr \/>\n<\/div><\/div><\/div><\/div><div id=\"pg-1707-4\"  class=\"panel-grid panel-no-style\"  style=\"margin-bottom: 50px\" ><div id=\"pgc-1707-4-0\"  class=\"panel-grid-cell\" ><div id=\"panel-1707-4-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"4\" ><div class=\"textwidget\"><h2 style=\"text-align: center;\">Otimiza\u00e7\u00e3o de m\u00e9todos de elui\u00e7\u00e3o para purifica\u00e7\u00e3o de prote\u00ednas<\/h2>\n<p>A escolha do tamp\u00e3o de elui\u00e7\u00e3o correto, em cromatografia l\u00edquida para purifica\u00e7\u00e3o de enzimas, pode melhorar consideravelmente a qualidade da separa\u00e7\u00e3o e o tempo de an\u00e1lise.<\/p>\n<\/div><\/div><\/div><\/div><div id=\"pg-1707-5\"  class=\"panel-grid panel-has-style\"  style=\"margin-bottom: 50px\" ><div class=\"siteorigin-panels-stretch panel-row-style-full-width panel-row-style panel-row-style-for-1707-5\" style=\"background-color: #f7f7f7; \" data-stretch-type=\"full\" ><div id=\"pgc-1707-5-0\"  class=\"panel-grid-cell\" ><div id=\"panel-1707-5-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"5\" ><div style=\"padding: 25px 25px 25px 25px;\" class=\"panel-widget-style panel-widget-style-for-1707-5-0-0\" ><div class=\"textwidget\"><h3>Elui\u00e7\u00e3o isocr\u00e1tica em cromatografia em coluna l\u00edquida<\/h3>\n<p>Na cromatografia l\u00edquida, as prote\u00ednas s\u00e3o separadas sob <strong>composi\u00e7\u00e3o de solvente constante<\/strong> (ou seja, elui\u00e7\u00e3o isocr\u00e1tica):<\/p>\n<ul>\n<li>Quando as amostras n\u00e3o s\u00e3o muito complexas e tamb\u00e9m quando os componentes da amostra n\u00e3o t\u00eam tempos de reten\u00e7\u00e3o semelhantes.<\/li>\n<\/ul>\n<p>Al\u00e9m disso, para as prote\u00ednas mais fortemente adsorvidas, a elui\u00e7\u00e3o com composi\u00e7\u00e3o constante da fase m\u00f3vel ir\u00e1 <b>demoram muito tempo para serem elu\u00eddos<\/b>.<\/p>\n<p>Utilizam-se tamp\u00e3o e bomba perist\u00e1ltica para elui\u00e7\u00e3o isocr\u00e1tica.<\/p>\n<\/div><\/div><\/div><\/div><div id=\"pgc-1707-5-1\"  class=\"panel-grid-cell\" ><div id=\"panel-1707-5-1-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"6\" ><div style=\"padding: 25px 25px 25px 25px;\" class=\"panel-widget-style panel-widget-style-for-1707-5-1-0\" ><div class=\"textwidget\"><h3>Elui\u00e7\u00e3o em gradiente - para purifica\u00e7\u00e3o de prote\u00ednas a partir de misturas complexas (amostras biol\u00f3gicas)<\/h3>\n<p>Na elui\u00e7\u00e3o em gradiente, a composi\u00e7\u00e3o do <strong>mudan\u00e7as de fase m\u00f3vel<\/strong> durante o processo de separa\u00e7\u00e3o e influencia a reten\u00e7\u00e3o dos analitos.<\/p>\n<p>A elui\u00e7\u00e3o em gradiente \u00e9 vantajosamente utilizada na purifica\u00e7\u00e3o de prote\u00ednas:<\/p>\n<ul>\n<li><strong>amostras biol\u00f3gicas complexas<\/strong><\/li>\n<li><strong>M<\/strong><b>misturas com propriedades conhecidas<\/b><\/li>\n<li><strong>C<\/strong><b>componentes com tempos de reten\u00e7\u00e3o semelhantes<\/b><\/li>\n<\/ul>\n<p>Se a elui\u00e7\u00e3o em gradiente for usada na cromatografia l\u00edquida em coluna para a purifica\u00e7\u00e3o de prote\u00ednas, ent\u00e3o\u00a0<strong>O tempo de reten\u00e7\u00e3o pode ser reduzido.<\/strong><\/p>\n<p><span data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.4.0.4\">A elui\u00e7\u00e3o em gradiente \u00e9 realizada com\u00a0<\/span><span data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.4.0.5\"><strong>dois buffers<\/strong> e <strong>uma ou duas bombas perist\u00e1lticas.<\/strong><\/span><\/p>\n<\/div><\/div><\/div><\/div><\/div><\/div><div id=\"pg-1707-6\"  class=\"panel-grid panel-no-style\"  style=\"margin-bottom: 50px\" ><div id=\"pgc-1707-6-0\"  class=\"panel-grid-cell\" ><div id=\"panel-1707-6-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"7\" ><div class=\"textwidget\"><h3 style=\"text-align: center;\">Elui\u00e7\u00e3o em gradiente e perfil de elui\u00e7\u00e3o<\/h3>\n<p>A elui\u00e7\u00e3o em gradiente implica que a composi\u00e7\u00e3o da fase m\u00f3vel ser\u00e1 variada durante a separa\u00e7\u00e3o da amostra para uma separa\u00e7\u00e3o ideal no menor tempo poss\u00edvel.<\/p>\n<p>Com a composi\u00e7\u00e3o vari\u00e1vel do eluente (elui\u00e7\u00e3o em gradiente), o fator de reten\u00e7\u00e3o e, consequentemente, o fator de capacidade podem ser influenciados.<\/p>\n<h4>Os perfis de gradiente:<\/h4>\n<p style=\"padding-left: 90px;\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-1939\" src=\"https:\/\/www.fractioncollector.info\/wp-content\/uploads\/Linear-gradient-elution-of-proteins.jpg\" alt=\"\" width=\"238\" height=\"212\" \/> <img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-1940\" src=\"https:\/\/www.fractioncollector.info\/wp-content\/uploads\/Segmented-gradient-elution-of-proteins.jpg\" alt=\"\" width=\"238\" height=\"212\" \/>\u00a0<img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-1947 size-full\" src=\"https:\/\/www.fractioncollector.info\/wp-content\/uploads\/Stepwise-gradient-elution-of-proteins.jpg\" alt=\"\" width=\"238\" height=\"212\" srcset=\"https:\/\/www.fractioncollector.info\/wp-content\/uploads\/Stepwise-gradient-elution-of-proteins.jpg 238w, https:\/\/www.fractioncollector.info\/wp-content\/uploads\/Stepwise-gradient-elution-of-proteins-150x134.jpg 150w\" sizes=\"auto, (max-width: 238px) 100vw, 238px\" \/><\/p>\n<p>Geralmente, prefere-se o uso de gradientes lineares; isso pode ser implementado em <strong>formas lineares, segmentadas, convexas e c\u00f4ncavas<\/strong>. Os gradientes n\u00e3o lineares oferecem diversas vantagens distintas, incluindo tempo de separa\u00e7\u00e3o reduzido, melhor resolu\u00e7\u00e3o da amostra e maior sensibilidade de detec\u00e7\u00e3o.<\/p>\n<\/div><\/div><\/div><\/div><div id=\"pg-1707-7\"  class=\"panel-grid panel-has-style\"  style=\"margin-bottom: 50px\" ><div class=\"siteorigin-panels-stretch panel-row-style-full-width panel-row-style panel-row-style-for-1707-7\" data-stretch-type=\"full\" ><div id=\"pgc-1707-7-0\"  class=\"panel-grid-cell\" ><div id=\"panel-1707-7-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"8\" ><div class=\"textwidget\"><p style=\"text-align: left;\"><a href=\"https:\/\/www.lambda-instruments.com\/peristaltic-pumps\/\" target=\"_blank\" rel=\"noopener\"><img loading=\"lazy\" decoding=\"async\" class=\"alignleft wp-image-1952\" src=\"https:\/\/www.fractioncollector.info\/wp-content\/uploads\/Compact-and-reliable-LAMBDA-lab-peristaltic-pumps.gif\" alt=\"\" width=\"493\" height=\"358\" \/><\/a><\/p>\n<\/div><\/div><\/div><div id=\"pgc-1707-7-1\"  class=\"panel-grid-cell\" ><div class=\"panel-cell-style panel-cell-style-for-1707-7-1\" ><div id=\"panel-1707-7-1-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"9\" ><div style=\"padding: 25px 25px 25px 25px;\" class=\"panel-widget-style panel-widget-style-for-1707-7-1-0\" ><div class=\"textwidget\"><p style=\"text-align: left;\">\n<\/p><p style=\"text-align: left;\"><em>Bioqu\u00edmicos recomendam <strong>bombas perist\u00e1lticas LAMBDA para l\u00edquidos<\/strong> que garantem a aplica\u00e7\u00e3o da fase m\u00f3vel sem pulsa\u00e7\u00f5es: <a href=\"https:\/\/www.lambda-instruments.com\/peristaltic-pumps\/\" target=\"_blank\" rel=\"noopener\">www.lambda-instruments.com\/bombas-peristalticas\/<\/a>.<br \/>\n<\/em><\/p>\n<\/div><\/div><\/div><\/div><\/div><\/div><\/div><div id=\"pg-1707-8\"  class=\"panel-grid panel-no-style\"  style=\"margin-bottom: 50px\" ><div id=\"pgc-1707-8-0\"  class=\"panel-grid-cell\" ><div id=\"panel-1707-8-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"10\" ><div class=\"textwidget\"><h3 style=\"text-align: center;\">Gera\u00e7\u00e3o de gradientes com bombas perist\u00e1lticas confi\u00e1veis<\/h3>\n<p><span data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.0.0.0\">As bombas perist\u00e1lticas de laborat\u00f3rio LAMBDA s\u00e3o program\u00e1veis e control\u00e1veis remotamente para atingir os objetivos desejados.\u00a0<\/span><strong>elui\u00e7\u00e3o por gradiente linear, gradual e segmentado<\/strong><span data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.0.0.2\">.<\/span><\/p>\n<\/div><\/div><\/div><\/div><div id=\"pg-1707-9\"  class=\"panel-grid panel-no-style\"  style=\"margin-bottom: 50px\" ><div id=\"pgc-1707-9-0\"  class=\"panel-grid-cell\" ><div id=\"panel-1707-9-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"11\" ><div style=\"padding: 25px 25px 25px 25px;\" class=\"panel-widget-style panel-widget-style-for-1707-9-0-0\" ><div class=\"textwidget\"><h3 class=\"font_4\" data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.d\"><span data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.d.0\">Elui\u00e7\u00e3o em gradiente com uma \u00fanica bomba<\/span><\/h3>\n<p class=\"font_8\" data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.f\"><span data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.f.0\">A elui\u00e7\u00e3o em gradiente tamb\u00e9m pode ser realizada com uma \u00fanica bomba perist\u00e1ltica: os recipientes com\u00a0<strong>tamp\u00e3o de elui\u00e7\u00e3o A e B<\/strong> s\u00e3o <strong>conectados por um sif\u00e3o<\/strong>\u00a0ou tubo na parte inferior com uma v\u00e1lvula para ajustar o fluxo<\/span><\/p>\n<ol class=\"font_8\" data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.g\">\n<li data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.g.0\">\n<p class=\"font_8\" data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.g.0.0\"><span data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.g.0.0.0\">O tamp\u00e3o de elui\u00e7\u00e3o A \u00e9 aspirado de forma consistente para o b\u00e9quer com agita\u00e7\u00e3o constante contendo o tamp\u00e3o de elui\u00e7\u00e3o B, onde o\u00a0<strong>A concentra\u00e7\u00e3o do tamp\u00e3o est\u00e1 sendo alterada.<\/strong><\/span><\/p>\n<\/li>\n<li data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.g.1\">\n<p class=\"font_8\" data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.g.1.0\"><span data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.g.1.0.0\">Bomba perist\u00e1ltica LAMBDA program\u00e1vel usada para\u00a0<strong>bombeie a mistura gradiente<\/strong>\u00a0na coluna de cromatografia<\/span><\/p>\n<\/li>\n<li data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.g.2\">\n<p class=\"font_8\" data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.g.2.0\"><span data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.g.2.0.0\">O eluato da coluna passou pelo detector para medir o pH, a absorb\u00e2ncia a 280 nm e a condutividade.<\/span><\/p>\n<\/li>\n<li data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.g.3\">\n<p class=\"font_8\" data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.g.3.0\"><span data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.g.3.0.0\">A partir do detector, o\u00a0<strong>fracionamento do eluato coletado<\/strong>\u00a0pelo coletor de fra\u00e7\u00f5es OMNICOLL nos recipientes desejados\u00a0<em>(Exemplos: garrafas, b\u00e9queres, tubos, ampolas, etc.)<\/em><\/span><\/p>\n<\/li>\n<\/ol>\n<\/div><\/div><\/div><\/div><div id=\"pgc-1707-9-1\"  class=\"panel-grid-cell\" ><div id=\"panel-1707-9-1-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"12\" ><div style=\"padding: 25px 25px 25px 25px;\" class=\"panel-widget-style panel-widget-style-for-1707-9-1-0\" ><div class=\"textwidget\"><h3 class=\"font_4\" data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.8\"><span data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.8.0\">Elui\u00e7\u00e3o em gradiente com duas bombas<\/span><\/h3>\n<p class=\"font_8\" data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.a\"><span data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.a.0\"><strong>Duas bombas perist\u00e1lticas LAMBDA program\u00e1veis<\/strong>\u00a0S\u00e3o utilizadas para a mistura reprodut\u00edvel dos eluentes na elui\u00e7\u00e3o em gradiente.<\/span><\/p>\n<ol class=\"font_8\" data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.b\">\n<li data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.b.0\">\n<p class=\"font_8\" data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.b.0.0\"><span data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.b.0.0.0\">O <strong>mudan\u00e7a na composi\u00e7\u00e3o ao longo do tempo<\/strong> pode ser\u00a0<strong>programado<\/strong> diretamente no visor da bomba perist\u00e1ltica LAMBDA ou pode ser gravado e exibido como um gr\u00e1fico em tempo real usando o software de controle PNet para PC.<\/span><\/p>\n<\/li>\n<li data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.b.2\">\n<p class=\"font_8\" data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.b.2.0\"><span data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.b.2.0.0\">O\u00a0<strong>A mistura em gradiente passa pela coluna de cromatografia.<\/strong>\u00a0e os componentes s\u00e3o separados.<\/span><\/p>\n<\/li>\n<li data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.b.3\">\n<p class=\"font_8\" data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.b.3.0\"><span data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.b.3.0.0\">O eluato da coluna passou pelo detector para medir o\u00a0<strong>pH, absor\u00e7\u00e3o a 280 nm e condut\u00e2ncia<\/strong>.<\/span><\/p>\n<\/li>\n<li data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.b.4\">\n<p class=\"font_8\" data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.b.4.0\"><span data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.b.4.0.0\">Coletor autom\u00e1tico de fra\u00e7\u00f5es LAMBDA OMNICOLL,\u00a0<strong>fraciona o eluato<\/strong>\u00a0e recolhe as fra\u00e7\u00f5es com base no tempo programado ou no contador de gotas.<\/span><\/p>\n<\/li>\n<\/ol>\n<\/div><\/div><\/div><\/div><\/div><div id=\"pg-1707-10\"  class=\"panel-grid panel-no-style\"  style=\"margin-bottom: 50px\" ><div id=\"pgc-1707-10-0\"  class=\"panel-grid-cell\" ><div id=\"panel-1707-10-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child\" data-index=\"13\" style=\"margin-bottom: 50px\" ><div class=\"textwidget\"><hr \/>\n<p>\u00a0<\/p>\n<\/div><\/div><div id=\"panel-1707-10-0-1\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-last-child\" data-index=\"14\" ><div class=\"textwidget\"><h2 class=\"font_3\" style=\"text-align: center;\" data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.i\"><span data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.i.0\">Requisitos do sistema de bombeamento para cromatografia em coluna<\/span><\/h2>\n<\/div><\/div><\/div><\/div><div id=\"pg-1707-11\"  class=\"panel-grid panel-no-style\"  style=\"margin-bottom: 50px\" ><div id=\"pgc-1707-11-0\"  class=\"panel-grid-cell\" ><div id=\"panel-1707-11-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"15\" ><div class=\"textwidget\"><p class=\"font_8\" data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.k\"><span data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.k.0\">Os requisitos mais importantes do sistema de bombeamento para cromatografia em coluna s\u00e3o:<\/span><\/p>\n<ul class=\"font_8\" data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.l\">\n<li data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.l.0\">\n<p class=\"font_8\" data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.l.0.0\"><span data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.l.0.0.0\"><strong>Vaz\u00e3o constante e reproduz\u00edvel<\/strong><br data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.l.0.0.0.1\" \/>A reprodutibilidade depende dos tempos de reten\u00e7\u00e3o dos picos. Os tempos de reten\u00e7\u00e3o dependem da vaz\u00e3o.<\/span><\/p>\n<\/li>\n<li data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.l.1\">\n<p class=\"font_8\" data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.l.1.0\"><span data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.l.1.0.0\"><strong>Fluxo sem pulsa\u00e7\u00e3o<\/strong><br data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.l.1.0.0.1\" \/>A fase estacion\u00e1ria deve ser protegida contra picos de press\u00e3o. A linha de base do detector deve permanecer est\u00e1vel.<\/span><\/p>\n<\/li>\n<\/ul>\n<p>\u00a0<\/p>\n<p>As bombas perist\u00e1lticas program\u00e1veis LAMBDA MULTIFLOW, que v\u00eam com 5 anos de garantia, s\u00e3o recomendadas para obter taxas de fluxo reproduz\u00edveis.<\/p>\n<\/div><\/div><\/div><div id=\"pgc-1707-11-1\"  class=\"panel-grid-cell\" ><div id=\"panel-1707-11-1-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"16\" ><div class=\"textwidget\"><p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-1959\" src=\"https:\/\/www.fractioncollector.info\/wp-content\/uploads\/Peristaltic_pumps_LAMBDA.jpg\" alt=\"Bombas perist\u00e1lticas program\u00e1veis LAMBDA para laborat\u00f3rio\" width=\"394\" height=\"328\" srcset=\"https:\/\/www.fractioncollector.info\/wp-content\/uploads\/Peristaltic_pumps_LAMBDA.jpg 394w, https:\/\/www.fractioncollector.info\/wp-content\/uploads\/Peristaltic_pumps_LAMBDA-150x125.jpg 150w, https:\/\/www.fractioncollector.info\/wp-content\/uploads\/Peristaltic_pumps_LAMBDA-334x278.jpg 334w\" sizes=\"auto, (max-width: 394px) 100vw, 394px\" \/><\/p>\n<\/div><\/div><\/div><\/div><div id=\"pg-1707-12\"  class=\"panel-grid panel-no-style\"  style=\"margin-bottom: 50px\" ><div id=\"pgc-1707-12-0\"  class=\"panel-grid-cell\" ><div id=\"panel-1707-12-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"17\" ><div class=\"textwidget\"><h2 class=\"font_3\" style=\"text-align: center;\" data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.i\"><span data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.i.0\">Requisitos da fra\u00e7\u00e3o para purifica\u00e7\u00e3o de prote\u00ednas<\/span><\/h2>\n<\/div><\/div><\/div><\/div><div id=\"pg-1707-13\"  class=\"panel-grid panel-no-style\"  style=\"margin-bottom: 50px\" ><div id=\"pgc-1707-13-0\"  class=\"panel-grid-cell\" ><div id=\"panel-1707-13-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"18\" ><div class=\"textwidget\"><p class=\"font_8\" data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.k\"><span data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.k.0\">Os requisitos mais importantes do coletor de fra\u00e7\u00f5es para cromatografia em coluna l\u00edquida s\u00e3o:<\/span><\/p>\n<ul class=\"font_8\" data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.l\">\n<li data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.l.0\">\n<p class=\"font_8\" data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.l.0.0\"><span data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.l.0.0.0\"><strong>Capacidade irrestrita<\/strong><br data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.l.0.0.0.1\" \/>A possibilidade de coletar um n\u00famero ilimitado de fra\u00e7\u00f5es \u00e9 extremamente importante, especialmente durante a otimiza\u00e7\u00e3o do processo.<\/span><\/p>\n<\/li>\n<li data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.l.1\">\n<p class=\"font_8\" data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.l.1.0\"><span data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.l.1.0.0\"><strong>Faixa de volume diferente<\/strong><br data-reactid=\".0.$SITE_ROOT.$desktop_siteRoot.$PAGES_CONTAINER.$centeredContent.$inlineContent.$SITE_PAGES.$c1sew_DESKTOP.$inlineContent.$j0ky2w9u.$inlineContent.0.$child.$0.$inlineContent.$1.$5.$0.$richTextContainer.l.1.0.0.1\" \/>Para possibilitar a adapta\u00e7\u00e3o do coletor de fra\u00e7\u00f5es a diversos experimentos de laborat\u00f3rio e processos em escala piloto.<\/span><\/p>\n<\/li>\n<\/ul>\n<p>\u00a0<\/p>\n<p>O coletor de fra\u00e7\u00f5es personaliz\u00e1vel OMNICOLL \u00e9 ideal para a otimiza\u00e7\u00e3o eficiente de processos em escala laboratorial e piloto.<\/p>\n<p>\u00a0<\/p>\n<\/div><\/div><\/div><div id=\"pgc-1707-13-1\"  class=\"panel-grid-cell\" ><div id=\"panel-1707-13-1-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"19\" ><div class=\"textwidget\"><p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-928 size-full\" src=\"https:\/\/www.fractioncollector.info\/wp-content\/uploads\/figure3.8-1-Example-of-taking-4x2-fractions-in-a-4-channel-configuration.jpg\" alt=\"\" width=\"665\" height=\"684\" srcset=\"https:\/\/www.fractioncollector.info\/wp-content\/uploads\/figure3.8-1-Example-of-taking-4x2-fractions-in-a-4-channel-configuration.jpg 665w, https:\/\/www.fractioncollector.info\/wp-content\/uploads\/figure3.8-1-Example-of-taking-4x2-fractions-in-a-4-channel-configuration-146x150.jpg 146w, https:\/\/www.fractioncollector.info\/wp-content\/uploads\/figure3.8-1-Example-of-taking-4x2-fractions-in-a-4-channel-configuration-270x278.jpg 270w\" sizes=\"auto, (max-width: 665px) 100vw, 665px\" \/><\/p>\n<\/div><\/div><\/div><\/div><\/div>","protected":false},"excerpt":{"rendered":"<p>Protein purification: Liquid chromatography &amp; gradient elution with OMNICOLL fraction collector and LAMBDA peristaltic pumps For protein purification and isolation of peptides, nucleic acids or small molecules from the crude mixture of fermentation broth, liquid\u00a0column chromatography (LC) is widely used\u00a0with ion exchange, hydrophobic interaction, affinity or size exclusion in downstream processing (DSP).In order to optimize [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":2635,"comment_status":"closed","ping_status":"closed","sticky":true,"template":"","format":"standard","meta":{"ai_generated_summary":"","footnotes":""},"categories":[132,4],"tags":[131,126,128,127,14,125,130,129],"class_list":["post-1707","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-post-name","category-omnicoll","tag-automatic-fraction-collector","tag-gradient-elution","tag-linear-gradient-elution","tag-liquid-chromatography","tag-peristaltic-pump","tag-protein-purification","tag-segmented-stepwise-gradient-elution","tag-stepwise-gradient-elution","post-with-thumbnail","post-with-thumbnail-icon"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.2 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Successful Protein Purification: Gradient elution &#8211; LAMBDA OMNICOLL | LAMBDA Laboratory Instruments<\/title>\n<meta name=\"description\" content=\"Important aspects to consider while selecting the peristaltic pumps and fraction collector for gradient elution in liquid column chromatography.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.fractioncollector.info\/pt\/cromatografia-de-purificacao-de-proteinas-por-coletor-de-fracoes\/\" \/>\n<meta property=\"og:locale\" content=\"pt_PT\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Successful Protein Purification: Gradient elution &#8211; LAMBDA OMNICOLL | LAMBDA Laboratory Instruments\" \/>\n<meta property=\"og:description\" content=\"Important aspects to consider while selecting the peristaltic pumps and fraction collector for gradient elution in liquid column chromatography.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.fractioncollector.info\/pt\/cromatografia-de-purificacao-de-proteinas-por-coletor-de-fracoes\/\" \/>\n<meta property=\"og:site_name\" content=\"LAMBDA OMNICOLL | LAMBDA Laboratory Instruments\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/LambdaLaboratoryInstrumentsCzSro\/\" \/>\n<meta property=\"article:published_time\" content=\"2017-01-10T12:44:45+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2024-08-05T14:03:04+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.fractioncollector.info\/wp-content\/uploads\/OMNICOLL-fraction-collector-LAMBDA-pumps-protein-purification-gradient-elution.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"700\" \/>\n\t<meta property=\"og:image:height\" content=\"400\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"LAMBDA Laboratory Instruments\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@LAMBDA_Labinstr\" \/>\n<meta name=\"twitter:site\" content=\"@LAMBDA_Labinstr\" \/>\n<meta name=\"twitter:label1\" content=\"Escrito por\" \/>\n\t<meta name=\"twitter:data1\" content=\"LAMBDA Laboratory Instruments\" \/>\n\t<meta name=\"twitter:label2\" content=\"Tempo estimado de leitura\" \/>\n\t<meta name=\"twitter:data2\" content=\"7 minutos\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/www.fractioncollector.info\\\/fraction-collector-protein-purification-chromatography\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/www.fractioncollector.info\\\/fraction-collector-protein-purification-chromatography\\\/\"},\"author\":{\"name\":\"LAMBDA Laboratory Instruments\",\"@id\":\"https:\\\/\\\/www.fractioncollector.info\\\/#\\\/schema\\\/person\\\/a67b3904ca09f50b495f0b8ebc85ed56\"},\"headline\":\"Successful Protein Purification: Gradient elution\",\"datePublished\":\"2017-01-10T12:44:45+00:00\",\"dateModified\":\"2024-08-05T14:03:04+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/www.fractioncollector.info\\\/fraction-collector-protein-purification-chromatography\\\/\"},\"wordCount\":1108,\"publisher\":{\"@id\":\"https:\\\/\\\/www.fractioncollector.info\\\/#organization\"},\"image\":{\"@id\":\"https:\\\/\\\/www.fractioncollector.info\\\/fraction-collector-protein-purification-chromatography\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/www.fractioncollector.info\\\/wp-content\\\/uploads\\\/OMNICOLL-fraction-collector-LAMBDA-pumps-protein-purification-gradient-elution.jpg\",\"keywords\":[\"automatic fraction collector\",\"gradient elution\",\"linear gradient elution\",\"liquid chromatography\",\"peristaltic pump\",\"protein purification\",\"segmented stepwise gradient elution\",\"stepwise gradient elution\"],\"articleSection\":[\"Applications\",\"OMNICOLL\"],\"inLanguage\":\"pt-PT\"},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/www.fractioncollector.info\\\/fraction-collector-protein-purification-chromatography\\\/\",\"url\":\"https:\\\/\\\/www.fractioncollector.info\\\/fraction-collector-protein-purification-chromatography\\\/\",\"name\":\"Successful Protein Purification: Gradient elution &#8211; LAMBDA OMNICOLL | LAMBDA Laboratory Instruments\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/www.fractioncollector.info\\\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\\\/\\\/www.fractioncollector.info\\\/fraction-collector-protein-purification-chromatography\\\/#primaryimage\"},\"image\":{\"@id\":\"https:\\\/\\\/www.fractioncollector.info\\\/fraction-collector-protein-purification-chromatography\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/www.fractioncollector.info\\\/wp-content\\\/uploads\\\/OMNICOLL-fraction-collector-LAMBDA-pumps-protein-purification-gradient-elution.jpg\",\"datePublished\":\"2017-01-10T12:44:45+00:00\",\"dateModified\":\"2024-08-05T14:03:04+00:00\",\"description\":\"Important aspects to consider while selecting the peristaltic pumps and fraction collector for gradient elution in liquid column chromatography.\",\"breadcrumb\":{\"@id\":\"https:\\\/\\\/www.fractioncollector.info\\\/fraction-collector-protein-purification-chromatography\\\/#breadcrumb\"},\"inLanguage\":\"pt-PT\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/www.fractioncollector.info\\\/fraction-collector-protein-purification-chromatography\\\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"pt-PT\",\"@id\":\"https:\\\/\\\/www.fractioncollector.info\\\/fraction-collector-protein-purification-chromatography\\\/#primaryimage\",\"url\":\"https:\\\/\\\/www.fractioncollector.info\\\/wp-content\\\/uploads\\\/OMNICOLL-fraction-collector-LAMBDA-pumps-protein-purification-gradient-elution.jpg\",\"contentUrl\":\"https:\\\/\\\/www.fractioncollector.info\\\/wp-content\\\/uploads\\\/OMNICOLL-fraction-collector-LAMBDA-pumps-protein-purification-gradient-elution.jpg\",\"width\":700,\"height\":400,\"caption\":\"LAMBDA OMNICOLL fraction collector and LAMBDA peristaltic pumps for protein purification by concentration gradient elution\"},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/www.fractioncollector.info\\\/fraction-collector-protein-purification-chromatography\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\\\/\\\/www.fractioncollector.info\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Successful Protein Purification: Gradient elution\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/www.fractioncollector.info\\\/#website\",\"url\":\"https:\\\/\\\/www.fractioncollector.info\\\/\",\"name\":\"LAMBDA OMNICOLL\",\"description\":\"fraction collector &amp; auto-sampler\",\"publisher\":{\"@id\":\"https:\\\/\\\/www.fractioncollector.info\\\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/www.fractioncollector.info\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"pt-PT\"},{\"@type\":\"Organization\",\"@id\":\"https:\\\/\\\/www.fractioncollector.info\\\/#organization\",\"name\":\"LAMBDA Laboratory Instruments\",\"url\":\"https:\\\/\\\/www.fractioncollector.info\\\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"pt-PT\",\"@id\":\"https:\\\/\\\/www.fractioncollector.info\\\/#\\\/schema\\\/logo\\\/image\\\/\",\"url\":\"https:\\\/\\\/www.fractioncollector.info\\\/wp-content\\\/uploads\\\/lambda-logo.jpg\",\"contentUrl\":\"https:\\\/\\\/www.fractioncollector.info\\\/wp-content\\\/uploads\\\/lambda-logo.jpg\",\"width\":1599,\"height\":365,\"caption\":\"LAMBDA Laboratory Instruments\"},\"image\":{\"@id\":\"https:\\\/\\\/www.fractioncollector.info\\\/#\\\/schema\\\/logo\\\/image\\\/\"},\"sameAs\":[\"https:\\\/\\\/www.facebook.com\\\/LambdaLaboratoryInstrumentsCzSro\\\/\",\"https:\\\/\\\/x.com\\\/LAMBDA_Labinstr\",\"https:\\\/\\\/www.instagram.com\\\/lambdainstruments\\\/\",\"https:\\\/\\\/www.linkedin.com\\\/company\\\/lambda-laboratory-instruments?trk=biz-companies-cym\",\"https:\\\/\\\/www.pinterest.com\\\/lambdacz\\\/\",\"https:\\\/\\\/www.youtube.com\\\/user\\\/LambdaCZ\"]},{\"@type\":\"Person\",\"@id\":\"https:\\\/\\\/www.fractioncollector.info\\\/#\\\/schema\\\/person\\\/a67b3904ca09f50b495f0b8ebc85ed56\",\"name\":\"LAMBDA Laboratory Instruments\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Purifica\u00e7\u00e3o de prote\u00ednas bem-sucedida: elui\u00e7\u00e3o em gradiente \u2013 LAMBDA OMNICOLL | LAMBDA Laboratory Instruments","description":"Aspectos importantes a serem considerados na sele\u00e7\u00e3o de bombas perist\u00e1lticas e coletores de fra\u00e7\u00f5es para elui\u00e7\u00e3o em gradiente em cromatografia l\u00edquida em coluna.","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.fractioncollector.info\/pt\/cromatografia-de-purificacao-de-proteinas-por-coletor-de-fracoes\/","og_locale":"pt_PT","og_type":"article","og_title":"Successful Protein Purification: Gradient elution &#8211; LAMBDA OMNICOLL | LAMBDA Laboratory Instruments","og_description":"Important aspects to consider while selecting the peristaltic pumps and fraction collector for gradient elution in liquid column chromatography.","og_url":"https:\/\/www.fractioncollector.info\/pt\/cromatografia-de-purificacao-de-proteinas-por-coletor-de-fracoes\/","og_site_name":"LAMBDA OMNICOLL | LAMBDA Laboratory Instruments","article_publisher":"https:\/\/www.facebook.com\/LambdaLaboratoryInstrumentsCzSro\/","article_published_time":"2017-01-10T12:44:45+00:00","article_modified_time":"2024-08-05T14:03:04+00:00","og_image":[{"width":700,"height":400,"url":"https:\/\/www.fractioncollector.info\/wp-content\/uploads\/OMNICOLL-fraction-collector-LAMBDA-pumps-protein-purification-gradient-elution.jpg","type":"image\/jpeg"}],"author":"LAMBDA Laboratory Instruments","twitter_card":"summary_large_image","twitter_creator":"@LAMBDA_Labinstr","twitter_site":"@LAMBDA_Labinstr","twitter_misc":{"Escrito por":"LAMBDA Laboratory Instruments","Tempo estimado de leitura":"7 minutos"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/www.fractioncollector.info\/fraction-collector-protein-purification-chromatography\/#article","isPartOf":{"@id":"https:\/\/www.fractioncollector.info\/fraction-collector-protein-purification-chromatography\/"},"author":{"name":"LAMBDA Laboratory Instruments","@id":"https:\/\/www.fractioncollector.info\/#\/schema\/person\/a67b3904ca09f50b495f0b8ebc85ed56"},"headline":"Successful Protein Purification: Gradient elution","datePublished":"2017-01-10T12:44:45+00:00","dateModified":"2024-08-05T14:03:04+00:00","mainEntityOfPage":{"@id":"https:\/\/www.fractioncollector.info\/fraction-collector-protein-purification-chromatography\/"},"wordCount":1108,"publisher":{"@id":"https:\/\/www.fractioncollector.info\/#organization"},"image":{"@id":"https:\/\/www.fractioncollector.info\/fraction-collector-protein-purification-chromatography\/#primaryimage"},"thumbnailUrl":"https:\/\/www.fractioncollector.info\/wp-content\/uploads\/OMNICOLL-fraction-collector-LAMBDA-pumps-protein-purification-gradient-elution.jpg","keywords":["automatic fraction collector","gradient elution","linear gradient elution","liquid chromatography","peristaltic pump","protein purification","segmented stepwise gradient elution","stepwise gradient elution"],"articleSection":["Applications","OMNICOLL"],"inLanguage":"pt-PT"},{"@type":"WebPage","@id":"https:\/\/www.fractioncollector.info\/fraction-collector-protein-purification-chromatography\/","url":"https:\/\/www.fractioncollector.info\/fraction-collector-protein-purification-chromatography\/","name":"Purifica\u00e7\u00e3o de prote\u00ednas bem-sucedida: elui\u00e7\u00e3o em gradiente \u2013 LAMBDA OMNICOLL | LAMBDA Laboratory Instruments","isPartOf":{"@id":"https:\/\/www.fractioncollector.info\/#website"},"primaryImageOfPage":{"@id":"https:\/\/www.fractioncollector.info\/fraction-collector-protein-purification-chromatography\/#primaryimage"},"image":{"@id":"https:\/\/www.fractioncollector.info\/fraction-collector-protein-purification-chromatography\/#primaryimage"},"thumbnailUrl":"https:\/\/www.fractioncollector.info\/wp-content\/uploads\/OMNICOLL-fraction-collector-LAMBDA-pumps-protein-purification-gradient-elution.jpg","datePublished":"2017-01-10T12:44:45+00:00","dateModified":"2024-08-05T14:03:04+00:00","description":"Aspectos importantes a serem considerados na sele\u00e7\u00e3o de bombas perist\u00e1lticas e coletores de fra\u00e7\u00f5es para elui\u00e7\u00e3o em gradiente em cromatografia l\u00edquida em coluna.","breadcrumb":{"@id":"https:\/\/www.fractioncollector.info\/fraction-collector-protein-purification-chromatography\/#breadcrumb"},"inLanguage":"pt-PT","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.fractioncollector.info\/fraction-collector-protein-purification-chromatography\/"]}]},{"@type":"ImageObject","inLanguage":"pt-PT","@id":"https:\/\/www.fractioncollector.info\/fraction-collector-protein-purification-chromatography\/#primaryimage","url":"https:\/\/www.fractioncollector.info\/wp-content\/uploads\/OMNICOLL-fraction-collector-LAMBDA-pumps-protein-purification-gradient-elution.jpg","contentUrl":"https:\/\/www.fractioncollector.info\/wp-content\/uploads\/OMNICOLL-fraction-collector-LAMBDA-pumps-protein-purification-gradient-elution.jpg","width":700,"height":400,"caption":"LAMBDA OMNICOLL fraction collector and LAMBDA peristaltic pumps for protein purification by concentration gradient elution"},{"@type":"BreadcrumbList","@id":"https:\/\/www.fractioncollector.info\/fraction-collector-protein-purification-chromatography\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/www.fractioncollector.info\/"},{"@type":"ListItem","position":2,"name":"Successful Protein Purification: Gradient elution"}]},{"@type":"WebSite","@id":"https:\/\/www.fractioncollector.info\/#website","url":"https:\/\/www.fractioncollector.info\/","name":"LAMBDA \u00d4NICO","description":"coletor de fra\u00e7\u00f5es e amostrador autom\u00e1tico","publisher":{"@id":"https:\/\/www.fractioncollector.info\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/www.fractioncollector.info\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"pt-PT"},{"@type":"Organization","@id":"https:\/\/www.fractioncollector.info\/#organization","name":"Instrumentos de laborat\u00f3rio LAMBDA","url":"https:\/\/www.fractioncollector.info\/","logo":{"@type":"ImageObject","inLanguage":"pt-PT","@id":"https:\/\/www.fractioncollector.info\/#\/schema\/logo\/image\/","url":"https:\/\/www.fractioncollector.info\/wp-content\/uploads\/lambda-logo.jpg","contentUrl":"https:\/\/www.fractioncollector.info\/wp-content\/uploads\/lambda-logo.jpg","width":1599,"height":365,"caption":"LAMBDA Laboratory Instruments"},"image":{"@id":"https:\/\/www.fractioncollector.info\/#\/schema\/logo\/image\/"},"sameAs":["https:\/\/www.facebook.com\/LambdaLaboratoryInstrumentsCzSro\/","https:\/\/x.com\/LAMBDA_Labinstr","https:\/\/www.instagram.com\/lambdainstruments\/","https:\/\/www.linkedin.com\/company\/lambda-laboratory-instruments?trk=biz-companies-cym","https:\/\/www.pinterest.com\/lambdacz\/","https:\/\/www.youtube.com\/user\/LambdaCZ"]},{"@type":"Person","@id":"https:\/\/www.fractioncollector.info\/#\/schema\/person\/a67b3904ca09f50b495f0b8ebc85ed56","name":"Instrumentos de laborat\u00f3rio LAMBDA"}]}},"_links":{"self":[{"href":"https:\/\/www.fractioncollector.info\/pt\/wp-json\/wp\/v2\/posts\/1707","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.fractioncollector.info\/pt\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.fractioncollector.info\/pt\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.fractioncollector.info\/pt\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.fractioncollector.info\/pt\/wp-json\/wp\/v2\/comments?post=1707"}],"version-history":[{"count":2,"href":"https:\/\/www.fractioncollector.info\/pt\/wp-json\/wp\/v2\/posts\/1707\/revisions"}],"predecessor-version":[{"id":4141,"href":"https:\/\/www.fractioncollector.info\/pt\/wp-json\/wp\/v2\/posts\/1707\/revisions\/4141"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.fractioncollector.info\/pt\/wp-json\/wp\/v2\/media\/2635"}],"wp:attachment":[{"href":"https:\/\/www.fractioncollector.info\/pt\/wp-json\/wp\/v2\/media?parent=1707"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.fractioncollector.info\/pt\/wp-json\/wp\/v2\/categories?post=1707"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.fractioncollector.info\/pt\/wp-json\/wp\/v2\/tags?post=1707"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}