{"id":1794,"date":"2017-04-04T13:42:00","date_gmt":"2017-04-04T11:42:00","guid":{"rendered":"https:\/\/www.fractioncollector.info\/?p=1794"},"modified":"2024-06-27T17:55:49","modified_gmt":"2024-06-27T15:55:49","slug":"perfusao-de-ilhotas-com-coletor-de-fracoes-automatizado-omnicoll","status":"publish","type":"post","link":"https:\/\/www.fractioncollector.info\/pt\/islets-perifusion-with-automated-fraction-collector-omnicoll\/","title":{"rendered":"Perfus\u00e3o de ilhotas com coletor de fra\u00e7\u00f5es automatizado"},"content":{"rendered":"

PERIFUS\u00c3O DE ILHOTAS: Coleta automatizada de fra\u00e7\u00f5es multicanal com LAMBDA OMNICOLL<\/span><\/strong><\/h1>\n<\/div><\/div><\/div><\/div><\/div>
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\n\t\t\t\t\t\tILHAS PANCRE\u00c1TICAS\t\t\t\t\t\t<\/h2>\n\t\t\t\t\t\t<\/div>\n<\/div><\/div><\/div>
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Ilhotas pancre\u00e1ticas<\/strong> Acredita-se que desempenhem um papel fundamental na fisiopatologia de Diabetes tipo 1 e tipo 2<\/strong> devido \u00e0 incapacidade das c\u00e9lulas \u03b2 das ilhotas pancre\u00e1ticas de secretarem insulina em quantidade suficiente para regular a glicemia.<\/p>\n

A medida definitiva da qualidade das c\u00e9lulas \u03b2 em uma ilhota \u00e9 a medi\u00e7\u00e3o da fun\u00e7\u00e3o das c\u00e9lulas \u03b2, ou seja, a capacidade das ilhotas de liberar insulina de forma controlada em resposta a m\u00ednimas altera\u00e7\u00f5es nos n\u00edveis de glicose no ambiente.<\/p>\n

A utiliza\u00e7\u00e3o de sistemas de perfus\u00e3o para compreender a cin\u00e9tica das c\u00e9lulas \u03b2 in vitro tem sido um fator chave na elucida\u00e7\u00e3o da fisiologia e fisiopatologia das ilhotas pancre\u00e1ticas. A perfus\u00e3o das ilhotas envolve a Exposi\u00e7\u00e3o das ilhotas a um fluxo cont\u00ednuo de nutrientes, gases ou reagentes.<\/strong>. Diversos pesquisadores publicaram sistemas de perfus\u00e3o com controle de temperatura, pH e taxa de fluxo do perfusato.<\/p>\n

Atualmente, n\u00e3o existe um aparato e protocolo padronizados e de baixo custo que possam ser replicados facilmente em qualquer laborat\u00f3rio.<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div>

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\n\t\t\t\t\t\tPERIFUS\u00c3O DAS ILHAS\t\t\t\t\t\t<\/h2>\n\t\t\t\t\t\t<\/div>\n<\/div><\/div><\/div>
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O Configura\u00e7\u00e3o da perfus\u00e3o das ilhotas<\/strong> consiste em quatro colunas de perfus\u00e3o<\/strong> conectado a quatro bombas Isamtec de canal \u00fanico, para bombear 4 solu\u00e7\u00f5es de perfus\u00e3o diferentes em 4 colunas paralelas contendo ilhotas simultaneamente com o vaz\u00e3o de 100 \u00b5L\/min<\/strong>. O fluxo cont\u00ednuo ou a perfus\u00e3o din\u00e2mica da solu\u00e7\u00e3o contendo glicose e secretagogos atrav\u00e9s das ilhotas \u00e9 a avalia\u00e7\u00e3o mais precisa da libera\u00e7\u00e3o regulada de insulina in vitro.<\/p>\n

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\u00c9 poss\u00edvel utilizar qualquer bomba perist\u00e1ltica de terceiros com sinais anal\u00f3gicos atrav\u00e9s do coletor de fra\u00e7\u00f5es multicanal automatizado LAMBDA OMNICOLL.\u00a0<\/em><\/p>\n<\/blockquote>\n

\"Perfus\u00e3o<\/p>\n

As colunas contendo as ilhotas e o meio de perfus\u00e3o foram mantidas a 37 \u00b0C. em uma c\u00e2mara com temperatura controlada.<\/strong> As ilhotas foram perfundidas durante 10 a 15 minutos por est\u00edmulo para permitir a exposi\u00e7\u00e3o adequada das ilhotas e a coleta de seu produto de secre\u00e7\u00e3o.<\/p>\n

Os perfusatos (100 \u03bcL\/min) de 4 colunas foram coletados simultaneamente a cada minuto por um sistema automatizado. Coletor de fra\u00e7\u00f5es de 4 canais LAMBDA OMNICOLL em placas de 96 po\u00e7os<\/strong>. O coletor de amostras LAMBDA OMNICOLL de 4 canais seleciona os 4 po\u00e7os superiores da placa de 96 po\u00e7os a cada minuto. (A, B, C e D - cada uma representando uma condi\u00e7\u00e3o de tratamento diferente)<\/em> Para coletar 12 minutos de fra\u00e7\u00f5es e depois passar para as quatro linhas inferiores. (Linhas E, F, G e H)<\/em> da placa de 96 po\u00e7os.<\/p>\n

O meio de perfus\u00e3o nas placas de coleta de 96 po\u00e7os foi mantido a uma temperatura inferior a 4 \u00b0C para preservar a integridade dos analitos.<\/p>\n

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As bases do coletor de fra\u00e7\u00f5es multicanal LAMBDA OMNICOLL (contendo as amostras de perfusato) podem ser colocadas em banho-maria frio ou em qualquer outro recipiente termoestabilizado.<\/em><\/p>\n<\/blockquote>\n

As amostras coletadas do perifuso foram analisadas para concentra\u00e7\u00e3o de insulina, glucagon ou somatostatina<\/strong>. A medi\u00e7\u00e3o dos diferentes horm\u00f4nios pode ser feita em diferentes po\u00e7os da mesma placa de 96 po\u00e7os.
\nEm princ\u00edpio, 96 amostras de prepara\u00e7\u00f5es de m\u00faltiplas ilhotas<\/strong> Diferentes condi\u00e7\u00f5es experimentais podem ser medidas simultaneamente, permitindo uma triagem de alto rendimento.<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div>

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Coleta automatizada de fra\u00e7\u00f5es multicanal com LAMBDA OMNICOLL configurada para placas de 96 po\u00e7os.<\/h2>\n

Para placas de 96 po\u00e7os, al\u00e9m disso coletores de fra\u00e7\u00f5es LAMBDA OMNICOLL automatizados multicanal<\/a> foi personalizado:<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div>

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\"Coletor<\/p>\n

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NOVO! Vers\u00e3o de configura\u00e7\u00e3o 2022<\/strong><\/a>
\nExemplo:<\/em> Coletor de fra\u00e7\u00f5es LAMBDA OMNICOLL personalizado de 6 canais para placas de 96 po\u00e7os.<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div>

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Outros exemplos: \"Coleta<\/p>\n

Cole\u00e7\u00e3o de fra\u00e7\u00f5es com 4 canais<\/strong> em placas de 96 po\u00e7os para perfus\u00e3o de ilhotas<\/p>\n<\/div>\n<\/div><\/div><\/div>

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\"Coletor<\/p>\n

Coletor de fra\u00e7\u00f5es multicanal automatizado para 6 fluxos simult\u00e2neos<\/strong> (1x6) em placas de 96 po\u00e7os<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div>

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\"Coletor<\/p>\n

Amostrador autom\u00e1tico multicanal LAMBDA OMNICOLL personalizado com 2 fluxos simult\u00e2neos<\/strong> e duas bombas LAMBDA<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div><\/div>","protected":false},"excerpt":{"rendered":"

PERIFUS\u00c3O DE ILHOTAS: Coleta automatizada de fra\u00e7\u00f5es multicanal com LAMBDA OMNICOLL<\/p>","protected":false},"author":2,"featured_media":1798,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"ai_generated_summary":"","footnotes":""},"categories":[132],"tags":[95,138,139,137,119,140],"class_list":["post-1794","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-post-name","tag-4-channel","tag-96-well-plates","tag-automated-fraction-collector","tag-islets-perifusion","tag-ismatec-multichannel-pump","tag-pancreatic-islets","post-with-thumbnail","post-with-thumbnail-icon"],"yoast_head":"\nIslets perifusion with automated fraction collector – LAMBDA OMNICOLL | LAMBDA Laboratory Instruments<\/title>\n<meta name=\"description\" content=\"Islets perifusion set-up with an automated 4-channel fraction collector OMNICOLL, to collect perifusate into 96-well plates\" \/>\n<meta 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