{"id":1771,"date":"2017-02-21T10:50:46","date_gmt":"2017-02-21T08:50:46","guid":{"rendered":"https:\/\/www.fractioncollector.info\/?p=1771"},"modified":"2023-10-04T15:51:26","modified_gmt":"2023-10-04T13:51:26","slug":"izolacia-a-charakterizacia-alelopatickych-zlucenin","status":"publish","type":"post","link":"https:\/\/www.fractioncollector.info\/sk\/isolation-and-characterization-of-allelopathic-compounds\/","title":{"rendered":"Izol\u00e1cia, \u010distenie a charakteriz\u00e1cia alelopatick\u00fdch zl\u00fa\u010den\u00edn"},"content":{"rendered":"

Izol\u00e1cia a charakteriz\u00e1cia alelopatick\u00fdch zl\u00fa\u010den\u00edn pomocou kol\u00f3novej chromatografie a frak\u010dn\u00e9ho zbera\u010da LAMBDA OMNICOLL<\/span><\/strong><\/h1>\n<\/div><\/div><\/div><\/div><\/div>

Izol\u00e1cia, objasnenie \u0161trukt\u00fary a chemick\u00e1 synt\u00e9za alelopatick\u00fdch zl\u00fa\u010den\u00edn<\/b> z rastl\u00edn, h\u00fab a in\u00fdch mikroorganizmov s\u00fa ve\u013emi pr\u00e1cne a \u010dasovo n\u00e1ro\u010dn\u00e9 \u0161t\u00fadie. Identifik\u00e1cia vysoko \u00fa\u010dinn\u00fdch biologick\u00fdch \u0161trukt\u00far v\u0161ak (pou\u017e\u00edvan\u00e9 ako pr\u00edrodn\u00e9 herbic\u00eddy, lie\u010div\u00e1, antioxidanty at\u010f.)<\/i> stoj\u00ed za ten \u010das a \u00fasilie vynalo\u017een\u00e9 na to.<\/p>\n

Rutinn\u00e1 \u0161t\u00fadia alelochemickej \u0161truktur\u00e1lnej anal\u00fdzy sa st\u00e1va n\u00e1ro\u010dnou, ke\u010f \u0161trukt\u00fary nie s\u00fa identifikovan\u00e9 v surovom extrakte, a to ani po nieko\u013ek\u00fdch predbe\u017en\u00fdch krokoch na dosiahnutie frakcion\u00e1cie surov\u00fdch extraktov. (macer\u00e1ciou, extrakciou pomocou ultrazvuku alebo mikrovlnn\u00e9ho \u017eiarenia, po ktorej nasleduj\u00fa dvojf\u00e1zov\u00e9 vodn\u00e9 extrakcie at\u010f.)<\/i>.<\/p>\n

V\u00fdskumn\u00edk potom zostane s ve\u013ek\u00fdm objemom rozp\u00fa\u0161\u0165adla, ktor\u00e9 m\u00f4\u017ee obsahova\u0165 \u010diasto\u010dn\u00fa zmes \u0161trukt\u00far (s podobn\u00fdmi biologick\u00fdmi aktivitami alebo bez nich)<\/i>. Biologick\u00e1 aktivita<\/b> \u0161trukt\u00fary mo\u017eno identifikova\u0165 pod\u013ea bioriaden\u00e1 \u0161t\u00fadia a testy<\/b>. Tento ve\u013ek\u00fd objem rozp\u00fa\u0161\u0165adla by sa mal rozdeli\u0165 na men\u0161ie frakcie pomocou zmes\u00ed rozp\u00fa\u0161\u0165adiel s r\u00f4zne polarity<\/b>. Zber frakci\u00ed pom\u00f4\u017ee izolova\u0165 najakt\u00edvnej\u0161ie alelopatick\u00e9 molekuly pre po\u017eadovan\u00fa\/cie\u013eov\u00fa biologick\u00fa aktivitu.<\/p>\n

V\u00e4\u010d\u0161inou, chromatografia na kvapalinovej kol\u00f3ne silikag\u00e9lu<\/b> sa pou\u017e\u00edva na frakcion\u00e1ciu extraktov. Be\u017ene sa v\u0161ak frakcionuj\u00fa a zhroma\u017e\u010fuj\u00fa elu\u00e1ty s hmotnos\u0165ou pribli\u017ene 65 \u2013 200 frakci\u00ed<\/b>, aby sa dosiahlo naj\u010distej\u0161ia bioakt\u00edvna \u0161trukt\u00fara v ka\u017edom elu\u00e1te<\/b>. Manu\u00e1lny zber ve\u013ek\u00e9ho po\u010dtu frakci\u00ed je \u010dasovo a energeticky najn\u00e1ro\u010dnej\u0161ou f\u00e1zou vo v\u0161eobecnom v\u00fdskumnom projekte kv\u00f4li vysok\u00fdm n\u00e1kladom na spektrofotometrick\u00e9 zbera\u010de frakci\u00ed na trhu.<\/p>\n

\u010eal\u0161ie kroky \u010distenia zah\u0155\u0148aj\u00fa tenkovrstvov\u00e1 chromatografia, bioakt\u00edvne testy, preparat\u00edvna kol\u00f3nov\u00e1 chromatografia <\/b>at\u010f. a nakoniec zoznam spektrometrick\u00e9 testy<\/b> pre vysoko akt\u00edvne \u0161trukt\u00fary, aby sa v bud\u00facnosti objasnila a dosiahla ich chemick\u00e1 synt\u00e9za.<\/p>\n

Ten\/T\u00e1\/To zber 200 alebo viacer\u00fdch frakci\u00ed je mo\u017en\u00e9 \u013eahko automatizova\u0165<\/b> najrealistickej\u0161\u00edm sp\u00f4sobom (ak je to spr\u00e1vne zbera\u010d frakci\u00ed<\/a> je vybran\u00fd!)<\/em>.<\/p>\n<\/div><\/div><\/div>

\"\"<\/a>

Izol\u00e1cia a \u010distenie alelopatick\u00fdch zl\u00fa\u010den\u00edn pomocou st\u013apcovej chromatografie na silikag\u00e9li, frakci\u00ed zhroma\u017eden\u00fdch v \u010dase pomocou zbera\u010da frakci\u00ed OMNICOLL a tenkovrstvovej chromatografie (TLC). \u010cisten\u00e9 zl\u00fa\u010deniny je mo\u017en\u00e9 charakterizova\u0165 UV, IR, LC-MS a NMR spektroskopick\u00fdmi \u0161t\u00fadiami.<\/p><\/div><\/p>\n<\/div><\/div><\/div><\/div>

Frakcion\u00e1cia surov\u00e9ho extraktu<\/h2>\n

Surov\u00e9 extrakty sa najprv rozdelia na r\u00f4zne samostatn\u00e9 frakcie obsahuj\u00face zl\u00fa\u010deniny s podobnou polaritou alebo molekulovou ve\u013ekos\u0165ou pomocou st\u013apcov\u00e1 chromatografia<\/b>. V tejto chromatografickej separ\u00e1cii pribli\u017ene Bolo zozbieran\u00fdch 65 frakci\u00ed s objemom 20 ml<\/b> (na z\u00e1klade \u010dasu) pod\u013ea zbera\u010d frakci\u00ed<\/b> OMNICOLL.<\/p>\n<\/div><\/div><\/div><\/div>

Tenkovrstvov\u00e1 chromatografia (TLC)<\/h2>\n

Alikvotn\u00e1 \u010das\u0165 v\u0161etk\u00fdch zozbieran\u00fdch frakci\u00ed bola nanesen\u00e1 na aktivovan\u00e9 silikag\u00e9lov\u00e9 TLC platne (20 x 20 cm). Frakcie boli zl\u00fa\u010den\u00e9 na z\u00e1klade vzor \u0161kv\u0155n a hodnoty Rf<\/b> na TLC platni. Spojen\u00e9 frakcie boli o\u010d\u00edslovan\u00e9 (Fr.1 - Fr.8).<\/p>\n<\/div><\/div><\/div><\/div>

Preparat\u00edvna TLC alebo HPLC st\u013apcov\u00e1 chromatografia<\/h2>\n

Frakcie (Fr.1 - Fr.8) sa m\u00f4\u017eu \u010falej \u010disti\u0165 pomocou preparat\u00edvna TLC a\/alebo HPLC st\u013apcov\u00e1 chromatografia<\/b>. Preparat\u00edvna HPLC pom\u00f4\u017ee presnej\u0161ie izolova\u0165 konkr\u00e9tnu zl\u00fa\u010deninu.<\/p>\n<\/div><\/div><\/div><\/div>

Charakteriz\u00e1cia alelopatick\u00fdch zl\u00fa\u010den\u00edn<\/h2>\n

Izolovan\u00e9 \u010dist\u00e9 frakcie sa potom pou\u017eij\u00fa na stanovenie \u0161trukt\u00fary a tie\u017e na op\u00e4tovn\u00e9 overenie biologickej aktivity.<\/p>\n

Charakteriz\u00e1cia \u010dist\u00fdch alelopatick\u00fdch zl\u00fa\u010den\u00edn sa vykon\u00e1va met\u00f3dou s vysok\u00fdm rozl\u00ed\u0161en\u00edm IR, MS, 1<\/sup>H a 13<\/sup>C NMR <\/b>spektr\u00e1.<\/p>\n

IR spektr\u00e1 sa pou\u017e\u00edvaj\u00fa na ur\u010denie funk\u010dn\u00fdch skup\u00edn izolovanej \u010distej frakcie. LC-MS poskytuje bu\u010f empirick\u00fa, alebo presn\u00fa (HRMS) molekulov\u00fa hmotnos\u0165 a NMR spektr\u00e1 odha\u013euj\u00fa prostredia C a H. LC-MS anal\u00fdza pom\u00e1ha porovna\u0165 MS obrazce.<\/p>\n<\/div><\/div><\/div><\/div><\/div>","protected":false},"excerpt":{"rendered":"

Isolation and characterization of allelopathic compounds by column chromatography and fraction collector LAMBDA OMNICOLL Isolation, structural elucidation and chemical synthesis of allelopathic compounds from plants, fungi, and other microorganisms are a very labor-exhausting and time-consuming studies. However, the identification of highly potent biological structures (used as natural herbicides, medicaments, antioxidants, etc.) is worth the time […]<\/p>\n","protected":false},"author":2,"featured_media":1789,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"ai_generated_summary":"","footnotes":""},"categories":[132,4],"tags":[135,134,6,136,133],"class_list":["post-1771","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-post-name","category-omnicoll","tag-allelopathic-compounds","tag-chemical-synthesis","tag-fraction-collector","tag-liquid-column-chromatography","tag-structural-elucidation","post-with-thumbnail","post-with-thumbnail-icon"],"yoast_head":"\nIsolation, purification and characterization of allelopathic compounds – LAMBDA OMNICOLL | LAMBDA Laboratory Instruments<\/title>\n<meta name=\"description\" content=\"Isolation of allelopathic compounds with silica gel column 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