{"id":1794,"date":"2017-04-04T13:42:00","date_gmt":"2017-04-04T11:42:00","guid":{"rendered":"https:\/\/www.fractioncollector.info\/?p=1794"},"modified":"2024-06-27T17:55:49","modified_gmt":"2024-06-27T15:55:49","slug":"otomatik-fraksiyon-toplayici-omnicoll-ile-adaciklarin-perfuzyonu","status":"publish","type":"post","link":"https:\/\/www.fractioncollector.info\/tr\/islets-perifusion-with-automated-fraction-collector-omnicoll\/","title":{"rendered":"Otomatik fraksiyon toplay\u0131c\u0131 ile adac\u0131klar\u0131n perf\u00fczyonu"},"content":{"rendered":"

ADACIK PER\u0130F\u00dcZYONU: LAMBDA OMNICOLL ile otomatik \u00e7ok kanall\u0131 fraksiyon toplama<\/span><\/strong><\/h1>\n<\/div><\/div><\/div><\/div><\/div>
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\n\t\t\t\t\t\tPANKREAS ADACIKLARI\t\t\t\t\t\t<\/h2>\n\t\t\t\t\t\t<\/div>\n<\/div><\/div><\/div>
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Pankreas adac\u0131klar\u0131<\/strong> patofizyolojisinde \u00f6nemli bir rol oynad\u0131\u011f\u0131 d\u00fc\u015f\u00fcn\u00fclmektedir. Tip 1 ve Tip 2 diyabet<\/strong> Pankreas adac\u0131klar\u0131ndaki \u03b2 h\u00fccrelerinin kan \u015fekerini d\u00fczenlemek i\u00e7in yeterli miktarda ins\u00fclin salg\u0131layamamas\u0131 nedeniyle.<\/p>\n

Pankreas adac\u0131klar\u0131ndaki \u03b2-h\u00fccre kalitesinin kesin \u00f6l\u00e7\u00fct\u00fc, \u03b2-h\u00fccre fonksiyonunun \u00f6l\u00e7\u00fclmesidir; yani, adac\u0131klar\u0131n ortamdaki glikoz seviyelerindeki k\u00fc\u00e7\u00fck de\u011fi\u015fikliklere yan\u0131t olarak ins\u00fclini kontroll\u00fc bir \u015fekilde salg\u0131layabilme yetene\u011fidir.<\/p>\n

\u0130n vitro ortamda \u03b2-h\u00fccre kineti\u011fini anlamak i\u00e7in perf\u00fczyon sistemlerinin kullan\u0131m\u0131, adac\u0131k fizyolojisi ve patofizyolojisinin ayd\u0131nlat\u0131lmas\u0131nda kilit bir fakt\u00f6r olmu\u015ftur. Adac\u0131k perf\u00fczyonu \u015funlar\u0131 i\u00e7erir: Adac\u0131klar\u0131n besin maddeleri, gaz veya reaktiflerle s\u00fcrekli ak\u0131\u015fa maruz kalmas\u0131<\/strong>. \u00c7e\u015fitli ara\u015ft\u0131rmac\u0131lar, perf\u00fczyon s\u0131v\u0131s\u0131n\u0131n s\u0131cakl\u0131\u011f\u0131n\u0131, pH'\u0131n\u0131 ve ak\u0131\u015f h\u0131z\u0131n\u0131 kontrol edebilen perf\u00fczyon sistemleri yay\u0131nlad\u0131lar.<\/p>\n

\u015eu anda herhangi bir laboratuvarda kolayca tekrarlanabilen, standartla\u015ft\u0131r\u0131lm\u0131\u015f, ucuz bir cihaz ve protokol bulunmamaktad\u0131r.<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div>

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\n\t\t\t\t\t\tADALAR PER\u0130F\u00dcZYONU\t\t\t\t\t\t<\/h2>\n\t\t\t\t\t\t<\/div>\n<\/div><\/div><\/div>
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O adac\u0131k perf\u00fczyon kurulumu<\/strong> \u015funlardan olu\u015fur d\u00f6rt perf\u00fczyon kolonu<\/strong> D\u00f6rt kanall\u0131 Isamtec pompalar\u0131na ba\u011fl\u0131 olarak, 4 farkl\u0131 perf\u00fczyon sol\u00fcsyonunu ayn\u0131 anda 4 paralel adac\u0131k i\u00e7eren kolona pompalamak i\u00e7in kullan\u0131l\u0131r. 100 \u00b5L\/dakika ak\u0131\u015f h\u0131z\u0131<\/strong>. Glukoz ve salg\u0131lat\u0131c\u0131 maddeler i\u00e7eren \u00e7\u00f6zeltinin adac\u0131klar i\u00e7inden s\u00fcrekli ak\u0131\u015f\u0131 veya dinamik perf\u00fczyonu, in vitro ortamda d\u00fczenlenmi\u015f ins\u00fclin sal\u0131n\u0131m\u0131n\u0131n en do\u011fru de\u011ferlendirme y\u00f6ntemidir.<\/p>\n

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LAMBDA OMNICOLL otomatik \u00e7ok kanall\u0131 fraksiyon toplay\u0131c\u0131 ile analog sinyalli herhangi bir \u00fc\u00e7\u00fcnc\u00fc taraf peristaltik pompa kullan\u0131labilir.\u00a0<\/em><\/p>\n<\/blockquote>\n

\"Otomatik<\/p>\n

Adac\u0131klar\u0131 bar\u0131nd\u0131ran kolonlar ve perf\u00fczyon ortam\u0131 37 \u00b0C'de tutuldu. S\u0131cakl\u0131k kontroll\u00fc bir odada.<\/strong> Adac\u0131klar\u0131n uygun \u015fekilde maruz kalmas\u0131n\u0131 ve salg\u0131 \u00fcr\u00fcnlerinin toplanmas\u0131n\u0131 sa\u011flamak i\u00e7in, her bir uyar\u0131 i\u00e7in 10-15 dakika s\u00fcreyle adac\u0131klara perf\u00fczyon uyguland\u0131.<\/p>\n

4 kolondan gelen perif\u00fczatlar (100 \u03bcL\/min), otomatik bir sistem taraf\u0131ndan her dakika e\u015f zamanl\u0131 olarak topland\u0131. 4 kanall\u0131 fraksiyon toplay\u0131c\u0131 LAMBDA OMNICOLL ile 96 kuyucuklu plakalara aktar\u0131m.<\/strong>. LAMBDA OMNICOLL 4 kanall\u0131 numune toplay\u0131c\u0131, 96 kuyucuklu plakan\u0131n en \u00fcstteki 4 kuyucu\u011funu her dakika se\u00e7er. (A, B, C ve D - her biri farkl\u0131 bir tedavi ko\u015fulunu temsil eder)<\/em> 12 dakika boyunca kesirleri toplay\u0131n ve ard\u0131ndan en alttaki d\u00f6rt sat\u0131ra ge\u00e7in. (E, F, G ve H sat\u0131rlar\u0131)<\/em> 96 kuyucuklu plakan\u0131n.<\/p>\n

96 kuyucuklu toplama plakalar\u0131ndaki perf\u00fczyon s\u0131v\u0131s\u0131, analiz edilen maddelerin b\u00fct\u00fcnl\u00fc\u011f\u00fcn\u00fc korumak i\u00e7in <4 \u00b0C'nin alt\u0131nda tutuldu.<\/p>\n

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LAMBDA OMNICOLL \u00e7ok kanall\u0131 fraksiyon toplay\u0131c\u0131 tabanlar\u0131 (perif\u00fczat \u00f6rneklerini i\u00e7eren) so\u011fuk banyoya veya herhangi bir ba\u015fka \u0131s\u0131ya dayan\u0131kl\u0131 kaba yerle\u015ftirilebilir.<\/em><\/p>\n<\/blockquote>\n

Toplanan perf\u00fczyon s\u0131v\u0131s\u0131 \u00f6rnekleri, a\u015fa\u011f\u0131daki ama\u00e7larla analiz edildi: ins\u00fclin, glukagon veya somatostatin konsantrasyonu<\/strong>. Farkl\u0131 hormonlar\u0131n \u00f6l\u00e7\u00fcm\u00fc, ayn\u0131 96 kuyucuklu plakan\u0131n farkl\u0131 kuyucuklar\u0131nda yap\u0131labilir.
\nPrensip olarak, 96 \u00f6rnekten \u00e7oklu adac\u0131k haz\u0131rl\u0131klar\u0131<\/strong> Farkl\u0131 deneysel ko\u015fullar alt\u0131nda e\u015f zamanl\u0131 \u00f6l\u00e7\u00fcmler yap\u0131labilmesi, y\u00fcksek verimli tarama olana\u011f\u0131 sa\u011flar.<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div>

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96 kuyucuklu plakalar i\u00e7in yap\u0131land\u0131r\u0131lm\u0131\u015f LAMBDA OMNICOLL ile otomatik \u00e7ok kanall\u0131 fraksiyon toplama<\/h2>\n

96 kuyucuklu plakalar i\u00e7in ayr\u0131ca otomatik \u00e7ok kanall\u0131 LAMBDA OMNICOLL fraksiyon toplay\u0131c\u0131lar\u0131<\/a> \u00f6zelle\u015ftirilmi\u015f:<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div>

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\"96<\/p>\n

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YEN\u0130! Yap\u0131land\u0131rma S\u00fcr\u00fcm\u00fc 2022<\/strong><\/a>
\n\u00d6rnek:<\/em> 96 kuyucuklu plakalar i\u00e7in \u00f6zelle\u015ftirilmi\u015f 6 kanall\u0131 fraksiyon toplay\u0131c\u0131 LAMBDA OMNICOLL.<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div>

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Di\u011fer \u00f6rnekler: \"Adac\u0131k<\/p>\n

Kesir koleksiyonu ile 4 kanal<\/strong> adac\u0131k perf\u00fczyonu i\u00e7in 96 kuyucuklu plakalara<\/p>\n<\/div>\n<\/div><\/div><\/div>

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\"96<\/p>\n

Otomatik \u00e7ok kanall\u0131 fraksiyon toplay\u0131c\u0131 6 e\u015f zamanl\u0131 ak\u0131\u015f<\/strong> (1x6) 96 kuyucuklu plakalara<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div>

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\"\u0130ki<\/p>\n

\u00d6zelle\u015ftirilmi\u015f LAMBDA OMNICOLL \u00e7ok kanall\u0131 otomatik \u00f6rnekleyici ile 2 e\u015f zamanl\u0131 ak\u0131\u015f<\/strong> ve iki LAMBDA pompas\u0131<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div><\/div>","protected":false},"excerpt":{"rendered":"

ADACIK PER\u0130F\u00dcZYONU: LAMBDA OMNICOLL ile otomatik \u00e7ok kanall\u0131 fraksiyon toplama<\/p>","protected":false},"author":2,"featured_media":1798,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"ai_generated_summary":"","footnotes":""},"categories":[132],"tags":[95,138,139,137,119,140],"class_list":["post-1794","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-post-name","tag-4-channel","tag-96-well-plates","tag-automated-fraction-collector","tag-islets-perifusion","tag-ismatec-multichannel-pump","tag-pancreatic-islets","post-with-thumbnail","post-with-thumbnail-icon"],"yoast_head":"\nIslets perifusion with automated fraction collector – LAMBDA OMNICOLL | LAMBDA Laboratory Instruments<\/title>\n<meta name=\"description\" content=\"Islets perifusion set-up with an automated 4-channel fraction collector OMNICOLL, to collect perifusate into 96-well plates\" \/>\n<meta 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