{"id":1771,"date":"2017-02-21T10:50:46","date_gmt":"2017-02-21T08:50:46","guid":{"rendered":"https:\/\/www.fractioncollector.info\/?p=1771"},"modified":"2023-10-04T15:51:26","modified_gmt":"2023-10-04T13:51:26","slug":"%e5%88%86%e7%a6%bb%e5%92%8c%e9%89%b4%e5%ae%9a%e5%8c%96%e6%84%9f%e5%8c%96%e5%90%88%e7%89%a9","status":"publish","type":"post","link":"https:\/\/www.fractioncollector.info\/zh\/isolation-and-characterization-of-allelopathic-compounds\/","title":{"rendered":"\u5316\u611f\u7269\u8d28\u7684\u5206\u79bb\u3001\u7eaf\u5316\u548c\u8868\u5f81"},"content":{"rendered":"

\u5229\u7528\u67f1\u8272\u8c31\u6cd5\u548c\u998f\u5206\u6536\u96c6\u5668\u5206\u79bb\u548c\u9274\u5b9a\u5316\u611f\u5316\u5408\u7269 LAMBDA OMNICOLL<\/span><\/strong><\/h1>\n<\/div><\/div><\/div><\/div><\/div>

\u5316\u611f\u5316\u5408\u7269\u7684\u5206\u79bb\u3001\u7ed3\u6784\u9274\u5b9a\u548c\u5316\u5b66\u5408\u6210<\/b> \u4ece\u690d\u7269\u3001\u771f\u83cc\u548c\u5176\u4ed6\u5fae\u751f\u7269\u4e2d\u9274\u5b9a\u9ad8\u6548\u751f\u7269\u7ed3\u6784\u662f\u4e00\u9879\u975e\u5e38\u8017\u65f6\u8017\u529b\u7684\u7814\u7a76\u3002\u7136\u800c\uff0c\u9274\u5b9a\u9ad8\u6548\u751f\u7269\u7ed3\u6784\u5374\u81f3\u5173\u91cd\u8981\u3002 \uff08\u53ef\u7528\u4f5c\u5929\u7136\u9664\u8349\u5242\u3001\u836f\u7269\u3001\u6297\u6c27\u5316\u5242\u7b49\uff09<\/i> \u503c\u5f97\u82b1\u8d39\u65f6\u95f4\u548c\u7cbe\u529b\u3002.<\/p>\n

\u5373\u4f7f\u7ecf\u8fc7\u51e0\u4e2a\u521d\u6b65\u6b65\u9aa4\u5bf9\u7c97\u63d0\u7269\u8fdb\u884c\u5206\u7ea7\u5206\u79bb\uff0c\u5982\u679c\u4ecd\u65e0\u6cd5\u5728\u7c97\u63d0\u7269\u4e2d\u9274\u5b9a\u51fa\u7ed3\u6784\uff0c\u90a3\u4e48\u5bf9\u5316\u611f\u7269\u8d28\u7ed3\u6784\u8fdb\u884c\u5e38\u89c4\u5206\u6790\u7684\u7814\u7a76\u5c31\u4f1a\u53d8\u5f97\u56f0\u96be\u3002 \uff08\u4f8b\u5982\uff0c\u901a\u8fc7\u6d78\u6e0d\u3001\u8d85\u58f0\u6ce2\u6216\u5fae\u6ce2\u8f85\u52a9\u8403\u53d6\uff0c\u7136\u540e\u8fdb\u884c\u6c34\u76f8\u53cc\u76f8\u8403\u53d6\u7b49\u65b9\u6cd5\uff09<\/i>.<\/p>\n

\u7814\u7a76\u4eba\u5458\u968f\u540e\u5c06\u63a5\u89e6\u5230\u5927\u91cf\u6eb6\u5242\uff0c\u5176\u4e2d\u53ef\u80fd\u542b\u6709\u90e8\u5206\u6df7\u5408\u7269\u7684\u7ed3\u6784\u3002 \uff08\u5177\u6709\u6216\u4e0d\u5177\u6709\u7c7b\u4f3c\u7684\u751f\u7269\u6d3b\u6027\uff09<\/i>. \u751f\u7269\u6d3b\u6027<\/b> \u8fd9\u4e9b\u7ed3\u6784\u53ef\u4ee5\u901a\u8fc7\u4ee5\u4e0b\u65b9\u5f0f\u8bc6\u522b\uff1a \u751f\u7269\u6307\u5bfc\u7814\u7a76\u548c\u68c0\u6d4b<\/b>. \u5982\u6b64\u5927\u91cf\u7684\u6eb6\u5242\u5e94\u8be5\u4f7f\u7528\u6eb6\u5242\u6df7\u5408\u7269\u5206\u6210\u8f83\u5c0f\u7684\u4efd\u91cf\u3002 \u4e0d\u540c\u6781\u6027<\/b>. \u6536\u96c6\u998f\u5206\u6709\u52a9\u4e8e\u5206\u79bb\u51fa\u5177\u6709\u6240\u9700\/\u76ee\u6807\u751f\u7269\u6d3b\u6027\u7684\u6700\u5177\u6d3b\u6027\u7684\u5316\u611f\u5206\u5b50\u3002.<\/p>\n

\u5927\u591a\u6570\u60c5\u51b5\u4e0b\uff0c \u7845\u80f6\u6db2\u76f8\u67f1\u8272\u8c31\u6cd5<\/b> \u7528\u4e8e\u63d0\u53d6\u7269\u7684\u5206\u7ea7\u5206\u79bb\u3002\u7136\u800c\uff0c\u901a\u5e38\u7684\u505a\u6cd5\u662f\u5206\u7ea7\u5206\u79bb\u5e76\u6536\u96c6\u7ea6 65 - 200 \u5206<\/b>, \u4e3a\u4e86\u5b9e\u73b0 \u6bcf\u4e2a\u6d17\u8131\u6db2\u4e2d\u6700\u7eaf\u51c0\u7684\u751f\u7269\u6d3b\u6027\u7ed3\u6784<\/b>. \u7531\u4e8e\u5e02\u573a\u4e0a\u5206\u5149\u5149\u5ea6\u998f\u5206\u6536\u96c6\u5668\u4ef7\u683c\u6602\u8d35\uff0c\u56e0\u6b64\u4eba\u5de5\u6536\u96c6\u5927\u91cf\u998f\u5206\u662f\u4e00\u822c\u7814\u7a76\u9879\u76ee\u4e2d\u8017\u65f6\u8017\u529b\u7684\u9636\u6bb5\u3002.<\/p>\n

\u8fdb\u4e00\u6b65\u7684\u7eaf\u5316\u6b65\u9aa4\u5305\u62ec \u8584\u5c42\u8272\u8c31\u6cd5\u3001\u751f\u7269\u6d3b\u6027\u6d4b\u5b9a\u3001\u5236\u5907\u67f1\u8272\u8c31\u6cd5 <\/b>\u7b49\u7b49\uff0c\u6700\u540e\uff0c\u5217\u51fa\u4e00\u4efd\u6e05\u5355 \u5149\u8c31\u5206\u6790<\/b> \u5bf9\u4e8e\u9ad8\u6d3b\u6027\u7ed3\u6784\uff0c\u4ee5\u4fbf\u5c06\u6765\u9610\u660e\u5e76\u5b9e\u73b0\u5176\u5316\u5b66\u5408\u6210\u3002.<\/p>\n

\u8fd9 \u6536\u96c6 200 \u4e2a\u6216\u66f4\u591a\u5206\u6570\u53ef\u4ee5\u8f7b\u677e\u5b9e\u73b0\u81ea\u52a8\u5316\u3002<\/b> \u4ee5\u6700\u53ef\u884c\u7684\u65b9\u5f0f \uff08\u5982\u679c\u5408\u9002\uff09 \u998f\u5206\u6536\u96c6\u5668<\/a> \u88ab\u9009\u4e2d\u4e86\uff01\uff09<\/em>.<\/p>\n<\/div><\/div><\/div>

\"\"<\/a>

\u91c7\u7528\u7845\u80f6\u67f1\u8272\u8c31\u6cd5\u3001OMNICOLL\u998f\u5206\u6536\u96c6\u5668\u6536\u96c6\u7684\u65f6\u95f4\u998f\u5206\u4ee5\u53ca\u8584\u5c42\u8272\u8c31\u6cd5(TLC)\u5206\u79bb\u7eaf\u5316\u5316\u611f\u5316\u5408\u7269\u3002\u7eaf\u5316\u540e\u7684\u5316\u5408\u7269\u53ef\u901a\u8fc7\u7d2b\u5916\u5149\u8c31(UV)\u3001\u7ea2\u5916\u5149\u8c31(IR)\u3001\u6db2\u76f8\u8272\u8c31-\u8d28\u8c31\u8054\u7528(LC-MS)\u548c\u6838\u78c1\u5171\u632f(NMR)\u5149\u8c31\u8fdb\u884c\u8868\u5f81\u3002.<\/p><\/div><\/p>\n<\/div><\/div><\/div><\/div>

\u7c97\u63d0\u7269\u7684\u5206\u7ea7\u5206\u79bb<\/h2>\n

\u9996\u5148\uff0c\u901a\u8fc7\u4ee5\u4e0b\u65b9\u6cd5\u5c06\u7c97\u63d0\u7269\u5206\u79bb\u6210\u5404\u79cd\u79bb\u6563\u7684\u7ec4\u5206\uff0c\u8fd9\u4e9b\u7ec4\u5206\u5305\u542b\u6781\u6027\u76f8\u4f3c\u6216\u5206\u5b50\u5927\u5c0f\u76f8\u8fd1\u7684\u5316\u5408\u7269\uff1a \u67f1\u8272\u8c31\u6cd5<\/b>. \u5728\u8fd9\u79cd\u8272\u8c31\u5206\u79bb\u4e2d\uff0c\u5927\u7ea6 \u6536\u96c6\u4e86 65 \u4efd\u4f53\u79ef\u5747\u4e3a 20 mL \u7684\u998f\u5206\u3002<\/b> \uff08\u57fa\u4e8e\u65f6\u95f4\uff09 \u998f\u5206\u6536\u96c6\u5668<\/b> OMNICOL\u3002.<\/p>\n<\/div><\/div><\/div><\/div>

\u8584\u5c42\u8272\u8c31\u6cd5\uff08TLC\uff09<\/h2>\n

\u5c06\u6240\u6709\u6536\u96c6\u7684\u998f\u5206\u53d6\u7b49\u5206\u8bd5\u6837\uff0c\u70b9\u6837\u4e8e\u6d3b\u5316\u7845\u80f6\u8584\u5c42\u8272\u8c31\u677f\uff0820 x 20 cm\uff09\u3002\u6839\u636e\u998f\u5206\u7684\u7ec4\u6210\u8fdb\u884c\u5408\u5e76\u3002 \u6591\u70b9\u6a21\u5f0f\u548c\u5c04\u9891\u503c<\/b> \u5728\u8584\u5c42\u8272\u8c31\u677f\u4e0a\u3002\u5408\u5e76\u7684\u998f\u5206\u7f16\u53f7\u4e3a\uff08Fr.1 - Fr.8\uff09\u3002.<\/p>\n<\/div><\/div><\/div><\/div>

\u5236\u5907\u578b\u8584\u5c42\u8272\u8c31\u6216\u9ad8\u6548\u6db2\u76f8\u8272\u8c31\u67f1\u8272\u8c31<\/h2>\n

\u998f\u5206\uff08Fr.1 - Fr.8\uff09\u53ef\u901a\u8fc7\u4ee5\u4e0b\u65b9\u6cd5\u8fdb\u4e00\u6b65\u7eaf\u5316\uff1a \u5236\u5907\u578b\u8584\u5c42\u8272\u8c31\u548c\/\u6216\u9ad8\u6548\u6db2\u76f8\u8272\u8c31\u67f1\u8272\u8c31<\/b>. \u5236\u5907\u578b\u9ad8\u6548\u6db2\u76f8\u8272\u8c31\u6cd5\u6709\u52a9\u4e8e\u66f4\u51c6\u786e\u5730\u5206\u79bb\u7279\u5b9a\u5316\u5408\u7269\u3002.<\/p>\n<\/div><\/div><\/div><\/div>

\u5316\u611f\u5316\u5408\u7269\u7684\u8868\u5f81<\/h2>\n

\u7136\u540e\u5229\u7528\u5206\u79bb\u5f97\u5230\u7684\u7eaf\u7ec4\u5206\u6765\u786e\u5b9a\u5176\u7ed3\u6784\uff0c\u5e76\u518d\u6b21\u68c0\u9a8c\u5176\u751f\u7269\u6d3b\u6027\u3002.<\/p>\n

\u91c7\u7528\u9ad8\u5206\u8fa8\u7387\u5149\u8c31\u6cd5\u5bf9\u7eaf\u5316\u611f\u7269\u8d28\u8fdb\u884c\u8868\u5f81\u3002 \u7ea2\u5916\u5149\u8c31\u3001\u8d28\u8c31\u3001, 1<\/sup>\u624b 13<\/sup>\u78b3\u8c31 <\/b>\u5149\u8c31\u3002.<\/p>\n

\u7ea2\u5916\u5149\u8c31\u7528\u4e8e\u786e\u5b9a\u5206\u79bb\u5f97\u5230\u7684\u7eaf\u7ec4\u5206\u7684\u5b98\u80fd\u56e2\u3002\u6db2\u76f8\u8272\u8c31-\u8d28\u8c31\u8054\u7528\uff08LC-MS\uff09\u53ef\u63d0\u4f9b\u7ecf\u9a8c\u5206\u5b50\u91cf\u6216\u7cbe\u786e\u5206\u5b50\u91cf\uff08\u9ad8\u5206\u8fa8\u8d28\u8c31HRMS\uff09\uff0c\u800c\u6838\u78c1\u5171\u632f\uff08NMR\uff09\u8c31\u56fe\u5219\u63ed\u793a\u78b3\u6c22\u5316\u5408\u7269\u7684\u5316\u5b66\u73af\u5883\u3002LC-MS\u5206\u6790\u6709\u52a9\u4e8e\u6bd4\u8f83\u8d28\u8c31\u56fe\u8c31\u3002.<\/p>\n<\/div><\/div><\/div><\/div><\/div>","protected":false},"excerpt":{"rendered":"

Isolation and characterization of allelopathic compounds by column chromatography and fraction collector LAMBDA OMNICOLL Isolation, structural elucidation and chemical synthesis of allelopathic compounds from plants, fungi, and other microorganisms are a very labor-exhausting and time-consuming studies. However, the identification of highly potent biological structures (used as natural herbicides, medicaments, antioxidants, etc.) is worth the time […]<\/p>\n","protected":false},"author":2,"featured_media":1789,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"ai_generated_summary":"","footnotes":""},"categories":[132,4],"tags":[135,134,6,136,133],"class_list":["post-1771","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-post-name","category-omnicoll","tag-allelopathic-compounds","tag-chemical-synthesis","tag-fraction-collector","tag-liquid-column-chromatography","tag-structural-elucidation","post-with-thumbnail","post-with-thumbnail-icon"],"yoast_head":"\nIsolation, purification and characterization of allelopathic compounds – LAMBDA OMNICOLL | LAMBDA Laboratory Instruments<\/title>\n<meta name=\"description\" content=\"Isolation of allelopathic compounds with silica gel column 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